Various lysates were subjected to SDS PAGE followed by western blot with 27599-1-AP (TREM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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1X10^6 BV-2 cells were intracellularly stained with 0.4 ug Anti-Human TREM2 (27599-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 68723-1-Ig (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

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Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 68723-1-Ig (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

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Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

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Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68723-1-PBS in a different storage buffer formulation.

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TREM2 fusion protein Ag30813 SDS-PAGE

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TREM2 fusion protein Ag30825 SDS-PAGE

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