TREM2 Monoclonal antibody

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  WB analysis using 68723-1-Ig

  Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 68723-1-Ig (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

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  IF Staining of mouse brain using 68723-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 68723-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 68723-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 68723-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TREM2 antibody (68723-1-Ig, Clone: 1F4H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TREM2 Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 68723-1-Ig

Clone No.1F4H10

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, CoIP, ELISA

Synonyms

Trem2c, Trem2b, Trem2a, TREM-2, TREM 2

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IF-P detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

68723-1-Ig targets TREM2 in WB, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6843

  Product name: Recombinant human TREM2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 22-222 aa of BC032362

  Sequence: TVFQGVAGQSLQVSCPYDSMKHWGRRKAWCRQLGEKGPCQRVVSTHNLWLLSFLRRWNGSTAITDDTLGGTLTITLRNLQPHDAGLYQCQSLHGSEADTLRKVLVEVLADPLDHRDAGDLWFPGESESFEDAHVEHSISRPSQGSHLPSCLSKEPLGRRNPLPTHFHPSPPGLHLSHQDSSSQRPLGCSLAWTEARDTSTQ

• Full Name: triggering receptor expressed on myeloid cells 2
• Calculated Molecular Weight: 222 aa, 25 kDa
• Observed Molecular Weight: 26-32 kDa
• GenBank Accession Number: BC032362
• Gene Symbol: TREM2
• Gene ID (NCBI): 54209
• RRID: AB_3206275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NZC2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Hepatol Int; A robust diagnostic model for high-risk MASH: integrating clinical parameters and circulating biomarkers through a multi-omics approach; Authors - Jie Zhang
mouse	IF	Front Neurosci; Perioperative enriched environment attenuates postoperative cognitive dysfunction by upregulating microglia TREM2 via PI3K/Akt pathway in mouse model of ischemic stroke; Authors - Yuchen Yao
	WB,CoIP	J Alzheimers Dis; IL-34/TREM2 modulates microglia-mediated inflammation and provides neuroprotection in a mouse model of sporadic Alzheimer's disease; Authors - Shi-Yao Wang