Tested Applications
| Positive WB detected in | THP-1 cells |
| Positive IF-P detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68723-1-Ig targets TREM2 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6843 Product name: Recombinant human TREM2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 22-222 aa of BC032362 Sequence: TVFQGVAGQSLQVSCPYDSMKHWGRRKAWCRQLGEKGPCQRVVSTHNLWLLSFLRRWNGSTAITDDTLGGTLTITLRNLQPHDAGLYQCQSLHGSEADTLRKVLVEVLADPLDHRDAGDLWFPGESESFEDAHVEHSISRPSQGSHLPSCLSKEPLGRRNPLPTHFHPSPPGLHLSHQDSSSQRPLGCSLAWTEARDTSTQ Predict reactive species |
| Full Name | triggering receptor expressed on myeloid cells 2 |
| Calculated Molecular Weight | 222 aa, 25 kDa |
| Observed Molecular Weight | 26-32 kDa |
| GenBank Accession Number | BC032362 |
| Gene Symbol | TREM2 |
| Gene ID (NCBI) | 54209 |
| RRID | AB_3206275 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NZC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TREM2 antibody 68723-1-Ig | Download protocol |
| WB protocol for TREM2 antibody 68723-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TREM2 antibody (Cat# 68723-1-Ig) has 17 citations across journals including Nature Aging, Advanced Science, Cancer Letters, Journal of Neuroinflammation, Cell Reports, Matter Today Bio, Hepatology International, Scientific Reports, Frontiers in Neuroscience, Journal of Alzheimer's Disease, and Environmental International. Research fields span Alzheimer's disease, neuroinflammation, microglia/macrophage biology, lipid metabolism, stroke, cancer metastasis, MASH, insulin resistance, myocardial infarction, ferroptosis, and environmental toxicology.
| Species | Application | Title |
|---|---|---|
Nat Aging Exercise alleviates cognitive dysfunction in Alzheimer's disease mice via skeletal muscle-derived extracellular vesicles that enhance plaque clearance by microglia | ||
J Adv Res Spatial-temporal lipidomics reveals dysregulated lipid metabolism in mouse brain during Alzheimer's disease progression | ||
Adv Sci (Weinh) TREM2-Mediated Cholesterol Efflux in Macrophages Inhibits Anti-Tumor Immunity via Limitation of CD4(+) T and NK Cells.
| ||
Cancer Lett Multi-omic profiling identifies TREM2(+) lipid-laden macrophages as inflammatory drivers and therapeutic targets of colorectal cancer liver metastasis. | ||
J Neuroinflammation Microbiota-derived IPA mitigates post-stroke neuroinflammation by inhibiting TREM2-dependent pyroptosis. | ||
Mater Today Bio Dual-functional hydrogel platform suppresses M1 activation and stabilizes M2 macrophages in intervertebral disc degeneration. |
Reviews
What customers say
One reviewer, Mitchell Wong, gave this product a 5-star rating in January 2026. He tested it for Western Blot, Immunofluorescence, Flow Cytometry, and Cell Culture using bone marrow-derived macrophages (lot 00133698) and described it as "an amazing product." Overall, the sole review is highly positive, indicating strong performance across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mitchell (Verified Customer) (01-30-2026) | This was an amazing product!
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