Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (66298-1-Ig, Clone: 1C10A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 Raji cells were stained with 0.20ug CD19 antibody (66298-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD19 (FITC-65197, Clone:4G7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD19 (FITC-65197, Clone:4G7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 0.2 ug Anti-Human CD19 (65197-1-Ig, Clone:4G7) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD19 (65197-1-Ig, Clone:4G7) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD19 Mouse Recombinant Antibody (65562-1-MR, Clone:4G7) or 0.5 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD19 Mouse Recombinant Antibody, PBS Only (65562-1-PBS, Clone:4G7) or 0.5 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD19 (65110-1-Ig, Clone:HIB19) or Mouse IgG1 Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500, then CL405 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD19 (65110-1-Ig, Clone:HIB19) or Mouse IgG1 Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD19 (65290-1-Ig, Clone:1D3) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 5 ul APC Anti-Human CD19 (APC-65110, Clone: HIB19) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD19 (APC-65110, Clone: HIB19) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.