Tested Applications
| Positive WB detected in | Raji cells, Ramos cells, Daudi cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66298-1-Ig targets CD19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19945 Product name: Recombinant human CD19 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 21-291 aa of BC006338 Sequence: EEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK Predict reactive species |
| Full Name | CD19 molecule |
| Calculated Molecular Weight | 556 aa, 61 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC006338 |
| Gene Symbol | CD19 |
| Gene ID (NCBI) | 930 |
| ENSEMBL Gene ID | ENSG00000177455 |
| RRID | AB_2881681 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P15391 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD19 antibody 66298-1-Ig | Download protocol |
| IHC protocol for CD19 antibody 66298-1-Ig | Download protocol |
| WB protocol for CD19 antibody 66298-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD19 Monoclonal antibody (1C10A1), catalog number 66298-1-Ig, has 18 citations. These appear in journals including Cancer Communications, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cancer Letters, Cell Reports, Cancer Cell International, Journal of Ethnopharmacology, Life Sciences, Inflammation Research, Journal of the American Heart Association, International Immunopharmacology, PLoS Pathogens, Journal of Gerontology, Functional & Integrative Genomics, Journal of Cellular Physiology, Frontiers in Medicine, and In Vitro Cell & Developmental Biology Animal. Research fields span oncology, immunology, rheumatology, cardiology, neurology, and aging.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis. | ||
J Exp Clin Cancer Res Investigating the impact of regulatory B cells and regulatory B cell-related genes on bladder cancer progression and immunotherapeutic sensitivity | ||
Adv Sci (Weinh) Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary-Patterned Malignancy in Lung Adenocarcinoma | ||
Cancer Lett Adenovirus-mediated specific tumor tagging facilitates CAR-T therapy against antigen-mismatched solid tumors. | ||
Cell Rep Dissociation of CCDC25 dimer into NETs-DNA-bound monomer licenses inflammatory activation of fibroblast-like synoviocytes in rheumatoid arthritis. | ||
Cancer Cell Int Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis. |
