CD19 Monoclonal antibody
CD19 Monoclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1C10A1
Cat no : 66298-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells, Ramos cells, Daudi cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IHC | See 2 publications below |
Product Information
66298-1-Ig targets CD19 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD19 fusion protein Ag19945 |
Full Name | CD19 molecule |
Calculated molecular weight | 556 aa, 61 kDa |
Observed molecular weight | 95 kDa |
GenBank accession number | BC006338 |
Gene symbol | CD19 |
Gene ID (NCBI) | 930 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD19 antibody 66298-1-Ig | Download protocol |
IHC protocol for CD19 antibody 66298-1-Ig | Download protocol |
FC protocol for CD19 antibody 66298-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Lett Adenovirus-mediated specific tumor tagging facilitates CAR-T therapy against antigen-mismatched solid tumors. | ||
Cancer Cell Int Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis. | ||
Int Immunopharmacol The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data | ||
In Vitro Cell Dev Biol Anim The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages. |