CD19 Monoclonal antibody

CD19 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1C10A1

Cat no : 66298-1-Ig

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Validation Data Gallery

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  WB analysis of Raji using 66298-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66298-1-Ig

  Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of Ramos using 66298-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66298-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Raji using 66298-1-Ig

  1X10^6 Raji cells were stained with 0.20ug CD19 antibody (66298-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

66298-1-Ig targets CD19 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD19 fusion protein Ag19945
• Full Name: CD19 molecule
• Calculated molecular weight: 556 aa, 61 kDa
• Observed molecular weight: 95 kDa
• GenBank accession number: BC006338
• Gene symbol: CD19
• Gene ID (NCBI): 930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

Publications

Species	Application	Title
human	WB	Cancer Lett; Adenovirus-mediated specific tumor tagging facilitates CAR-T therapy against antigen-mismatched solid tumors.; Authors - Xinying Tang
human	IHC	Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
human	IHC	Int Immunopharmacol; The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data; Authors - Xiaomin Lu
human	WB	In Vitro Cell Dev Biol Anim; The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages.; Authors - Liqiong Chen