CD19 Monoclonal antibody
CD19 Monoclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
WB, IHC, FC, ELISA
Cat no : 66298-1-Ig
Validation Data Gallery
|Positive WB detected in||Raji cells, Ramos cells, Daudi cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
66298-1-Ig targets CD19 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD19 fusion protein Ag19945|
|Full Name||CD19 molecule|
|Calculated molecular weight||556 aa, 61 kDa|
|Observed molecular weight||95 kDa|
|GenBank accession number||BC006338|
|Gene ID (NCBI)||930|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.
|Product Specific Protocols|
|WB protocol for CD19 antibody 66298-1-Ig||Download protocol|
|IHC protocol for CD19 antibody 66298-1-Ig||Download protocol|
|FC protocol for CD19 antibody 66298-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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