Validation Data Gallery
|Positive WB detected in
|Raji cells, Ramos cells, Daudi cells
|Positive IHC detected in
|human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:500-1:2000
|IF : 1:750-1:3000
|Flow Cytometry (FC)
|FC : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66298-1-Ig targets CD19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG1
|CD19 fusion protein Ag19945
|Calculated molecular weight
|556 aa, 61 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.
|Product Specific Protocols
|WB protocol for CD19 antibody 66298-1-Ig
|IHC protocol for CD19 antibody 66298-1-Ig
|IF protocol for CD19 antibody 66298-1-Ig
|FC protocol for CD19 antibody 66298-1-Ig
|Click here to view our Standard Protocols
Adenovirus-mediated specific tumor tagging facilitates CAR-T therapy against antigen-mismatched solid tumors.
Cancer Cell Int
Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.
The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data
In Vitro Cell Dev Biol Anim
The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages.
J Cell Physiol
Upregulation of ENKD1 disrupts cellular homeostasis to promote lymphoma development
Front Med (Lausanne)
Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL