Anti-Mouse CD19 (1D3)

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  IF Staining of mouse spleen using 65290-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD19 antibody (65290-1-Ig, Clone: 1D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of C57BL/6 mouse splenocytes using 65290-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD19 (65290-1-Ig, Clone:1D3) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

CD19 Monoclonal Antibody for IF-Fro, FC

Cat No. 65290-1-Ig

Clone No.1D3

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF-Fro, FC

Synonyms

CD19 antigen, 1D3

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Tested Applications

• Positive IF-Fro detected in: mouse spleen tissue
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65290-1-Ig targets CD19 in IF-Fro, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CD19 Transfected Cell Line
• Full Name: CD19 antigen
• GenBank Accession Number: BC156767
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• RRID: AB_3084916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).