Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 THP-1 cells untreated (dashed lines), LPS treated (red) were intracellularly stained with 0.4 ug Anti-Human IL-1 Beta (16806-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 THP-1 cells untreated (dashed lines) or treated with LPS (red) were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1 Beta (CL488-16806) , or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Standard curve of MP80001-1

Standard curve of MP80001-2

LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-6-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-6-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-6-PBS in a different storage buffer formulation.

Cytometric bead array standard curve of MP80001-1, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-6-RR. Detection antibody: 65276-1-PBS. Standard:HZ-1164. Range: 6.25-100 ng/mL.

Cytometric bead array standard curve of MP80001-2, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-6-RR. Detection antibody: 68767-1-PBS. Standard:HZ-1164. Range:3.125-100 ng/mL.