CoraLite® Plus 488-conjugated IL-1 Beta Polyclonal antibody
IL-1 Beta Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-16806
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | LPS treated THP-1 cells |
Recommended dilution
Application | Dilution |
---|---|
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL488-16806 is IL-1 Beta Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL-1 Beta fusion protein Ag10295 |
Full Name | interleukin 1, beta |
Calculated molecular weight | 269 aa, 31 kDa |
Observed molecular weight | 30-35 kDa |
GenBank accession number | BC008678 |
Gene symbol | IL1B |
Gene ID (NCBI) | 3553 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.
Protocols
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 IL-1 Beta antibody CL488-16806 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |