CoraLite® Plus 488-conjugated IL-1 Beta Polyclonal antibody

IL-1 Beta Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16806

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Validation Data Gallery

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  FC experiment of THP-1 using CL488-16806

  1X10^6 THP-1 cells untreated (dashed lines) or treated with LPS (red) were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1 Beta (CL488-16806) , or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: LPS treated THP-1 cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-16806 is IL-1 Beta Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-1 Beta fusion protein Ag10295
• Full Name: interleukin 1, beta
• Calculated molecular weight: 269 aa, 31 kDa
• Observed molecular weight: 30-35 kDa
• GenBank accession number: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.