Product name:

Human IL1-beta ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma, Cell culture supernatants

Assay type:

Sandwich

Sensitivity:

1.5 pg/mL

Range:

3.9-250 pg/mL

Reacted Species:

Human

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Human Plasma	101%	91%-117%
Cell culture supernatants	103%	83%-124%

Product overview:

KE00021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes human IL1-beta. A monoclonal antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:

Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta