Human IL-1 beta ELISA Kit
Cat no : KE00021
Synonyms
Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL1B, IL1beta, IL1F2, IL1β, Interleukin 1 beta, interleukin 1, beta
Validation Data Gallery
Product Information
KE00021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes human IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human IL-1 beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 101% | 91%-117% |
| Cell culture supernatants | 103% | 83%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 276.5 | 6.8 | 2.4 |
| 2 | 20 | 68.9 | 2.2 | 3.2 |
| 3 | 20 | 16.2 | 0.5 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 294.5 | 6.9 | 2.3 |
| 2 | 24 | 73.0 | 1.9 | 2.7 |
| 3 | 24 | 15.7 | 1.1 | 7.2 |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL1-beta and their naturally occurring inhibitor IL-1RN. Interleukin 1 beta (IL-1 beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1 beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms | Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL1B, IL1beta, IL1F2, IL1β, Interleukin 1 beta, interleukin 1, beta |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nutr Biochem Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats. | ||
Cell Death Dis LXRα promotes cell metastasis by regulating the NLRP3 inflammasome in renal cell carcinoma. | ||
Technol Cancer Res Treat Clinical Value of Serum and Exhaled Breath Condensate miR-186 and IL-1β Levels in Non-Small Cell Lung Cancer. | ||
Int J Ophthalmol Increased succinate receptor GPR91 involved in the pathogenesis of Mooren's ulcer. | ||
Exp Ther Med α-synuclein promotes progression of Parkinson's disease by upregulating autophagy signaling pathway to activate NLRP3 inflammasome. |