Human IL-1 beta ELISA Kit

Sensitivity

1.5 pg/mL

Range

3.9-250 pg/mL

Reacted Species

Human

Cat no : KE00021

Synonyms

Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL1B, IL1beta, IL1F2, IL1β, Interleukin 1 beta, interleukin 1, beta



Product Information

KE00021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes human IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human IL-1 beta ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.5 pg/mL
Range 3.9-250 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human Plasma101%91%-117%
Cell culture supernatants103%83%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120276.56.82.4
22068.92.23.2
32016.20.52.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124294.56.92.3
22473.01.92.7
32415.71.17.2

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL1-beta and their naturally occurring inhibitor IL-1RN. Interleukin 1 beta (IL-1 beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1 beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL1B, IL1beta, IL1F2, IL1β, Interleukin 1 beta, interleukin 1, beta

Publications

SpeciesSample TypeTitle
plasma

J Nutr Biochem

Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats.

Authors - Priscila Haygert
humancell culture supernatants

Cell Death Dis

LXRα promotes cell metastasis by regulating the NLRP3 inflammasome in renal cell carcinoma.

Authors - KeShan Wang
humancell culture supernatants

Front Oncol

Senolytics (DQ) Mitigates Radiation Ulcers by Removing Senescent Cells.

Authors - Huilan Wang
humanserum

Technol Cancer Res Treat

Clinical Value of Serum and Exhaled Breath Condensate miR-186 and IL-1β Levels in Non-Small Cell Lung Cancer.

Authors - Haiqin Xie
humancell culture supernatants

Int J Ophthalmol

Increased succinate receptor GPR91 involved in the pathogenesis of Mooren's ulcer.

Authors - Lin Li
humanserum

Exp Ther Med

α-synuclein promotes progression of Parkinson's disease by upregulating autophagy signaling pathway to activate NLRP3 inflammasome.

Authors - Xiaohong Wang