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Human IL1-beta ELISA Kit10 Publications

Catalog number: KE00021

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$399.00

Freight/Packing £00.00

Size: 09µ/gram

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Standard curve of KE00021 Human IL1-beta ELISA Kit

Overview

Product name:
Human IL1-beta ELISA Kit
Tests:
1 X 96 well plate
Sample type:
Serum, Plasma, Cell culture supernatants
Assay type:
Sandwich
Sensitivity:
1.5 pg/mL
Range:
3.9-250 pg/mL
Reacted Species:
Human
Tested applications:
Sandwich ELISA
Recovery:
Sample TypeAverageRange
Human Plasma 101% 91%-117%
Cell culture supernatants 103% 83%-124%
IntraAssay:
Samplenmean ( pg/mL)SDCV%
1 20 276.5 6.8 2.4
2 20 68.9 2.2 3.2
3 20 16.2 0.5 2.9
InterAssay:
Samplenmean ( pg/mL)SDCV%
1 24 294.5 6.9 2.3
2 24 73.0 1.9 2.7
3 24 15.7 1.1 7.2
Product overview:
KE00021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes human IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties

Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:
Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL1B, IL1beta, IL1F2, IL1β, Interleukin 1 beta, interleukin 1, beta
Background

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL1-beta and their naturally occurring inhibitor IL-1RN. Interleukin 1 beta (IL-1 beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1 beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.


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