Various lysates were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of PGC1a antibody (66369-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and PGC1a knockout HeLa cells.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®488 Anti-Human PGC1a (CL488-66369, Clone:1C1B2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 PGC1a antibody (CL488-66369, Clone: 1C1B2 ) at dilution of 1:400.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PGC1a (CL647-66369, Clone:1C1B2) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PGC1a antibody (CL594-66369, Clone: 1C1B2 ) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human PGC1a (CL594-66369, Clone:1C1B2) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Various lysates were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

WB result of PGC1a antibody (66369-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and PGC1a knockout HeLa cells. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.