• Featured Product
  • KD/KO Validated

PGC1a Monoclonal antibody

PGC1a Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig and More (6)







Cat no : 66369-1-Ig


LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PGC-1α, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A

Tested Applications

Positive WB detected inpig skeletal muscle tissue, C2C12 cells, HSC-T6 cells, ROS1728 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66369-1-Ig targets PGC1a in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, goat, chicken, ducks, monkey, zebrafish, hamster, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 70 kDa, 100 kDa
GenBank Accession NumberNM_013261
Gene Symbol PPARGC1A
Gene ID (NCBI) 10891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.


Product Specific Protocols
WB protocol for PGC1a antibody 66369-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen

Sci Adv

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Authors - Jun Seok Son

J Hazard Mater

Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers

Authors - Gaolong Zhong

J Autoimmun

Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis

Authors - Jean-Baptiste Vulsteke

Nat Commun

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Authors - Yan-Ting Chen

Nat Commun

METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice.

Authors - Yuqin Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Michaela (Verified Customer) (11-24-2023)

Worked well in 15μg mouse brain tissue lysate in Western Blot. Band appears between 60-100 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse brain tissue

No (Verified Customer) (10-24-2023)

A good antibody to detect endogenous PGC1a.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
PGC1a Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66369-1-Ig)

Mi (Verified Customer) (03-07-2023)

It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: adipocytes

Huai-Chin (Verified Customer) (07-11-2022)

There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: 3T3-L1 cells

Mohammad (Verified Customer) (01-21-2020)

It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse hippocampus