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PGC1a Monoclonal antibody

PGC1a Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 66369-1-Ig

Synonyms

LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PGC-1α, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A



Tested Applications

Positive WB detected inpig skeletal muscle tissue, C2C12 cells, HSC-T6 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66369-1-Ig targets PGC1a in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Calculated molecular weight 91 kDa
Observed molecular weight 100 kDa
GenBank accession numberNM_013261
Gene symbol PPARGC1A
Gene ID (NCBI) 10891
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

Protocols

Product Specific Protocols
WB protocol for PGC1a antibody 66369-1-IgDownload protocol
IF protocol for PGC1a antibody 66369-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Nutr Food Res

Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis in vivo and vitro.

Authors - Peng Lei
chickenWB

J Agric Food Chem

(-)-Hydroxycitric Acid Suppresses Lipid Droplet Accumulation and Accelerates Energy Metabolism via Activation of the Adiponectin-AMPK Signaling Pathway in Broiler Chickens.

Authors - Longlong Li
mouseWB

Nat Commun

The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.

Authors - Anton Iershov
mouseWB

Environ Int

Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice.

Authors - Xinming Shen
mouseWB

Biomolecules

Improvement of Mitochondrial Activity and Fibrosis by Resveratrol Treatment in Mice with Schistosoma japonicum Infection.

Authors - Tina Tuwen Chen
mouseWB

Sci Adv

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Authors - Jun Seok Son

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mohammad (Verified Customer) (01-21-2020)

It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse hippocampus