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- KD/KO Validated
PGC1a Monoclonal antibody
PGC1a Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG1
human, mouse, rat and More (5)
WB, IP, IHC, IF, ELISA
Cat no : 66369-1-Ig
|Positive WB detected in||pig skeletal muscle tissue, C2C12 cells, HSC-T6 cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 8 publications below|
|WB||See 205 publications below|
|IHC||See 12 publications below|
|IF||See 23 publications below|
|IP||See 2 publications below|
66369-1-Ig targets PGC1a in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, chicken, ducks, goat, pig|
|Host / Isotype||Mouse / IgG1|
|Full Name||peroxisome proliferator-activated receptor gamma, coactivator 1 alpha|
|Calculated molecular weight||91 kDa|
|Observed molecular weight||70 kDa, 100 kDa|
|GenBank accession number||NM_013261|
|Gene ID (NCBI)||10891|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.
|Product Specific Protocols|
|WB protocol for PGC1a antibody 66369-1-Ig||Download protocol|
|IF protocol for PGC1a antibody 66369-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mi (Verified Customer) (03-07-2023)
It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.
Huai-Chin (Verified Customer) (07-11-2022)
There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.
Mohammad (Verified Customer) (01-21-2020)
It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.