Tested Applications
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66369 targets PGC1a in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | peroxisome proliferator-activated receptor gamma, coactivator 1 alpha |
| Calculated Molecular Weight | 91 kDa |
| GenBank Accession Number | NM_013261 |
| Gene Symbol | PGC1a |
| Gene ID (NCBI) | 10891 |
| RRID | AB_2919318 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UBK2 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists a isoforms in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 PGC1a antibody CL488-66369 | Download protocol |
| IF protocol for CL Plus 488 PGC1a antibody CL488-66369 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CoraLite® Plus 488-conjugated PGC1a Monoclonal antibody (CL488-66369) has 3 citations. These appear in Frontiers in Immunology, FEBS Open Bio, and Journal of the Science of Food and Agriculture. The associated research fields span immunometabolism and innate lymphoid cell biology, mitochondrial integrity and AMPK signaling in neurodegeneration, and animal nutrition with antioxidant/metabolic studies in pigs. Applications cited include immunofluorescence (IF) and Western blot (WB) across mouse, human, and pig species.
| Species | Application | Title |
|---|---|---|
Front Immunol Immunometabolic analysis of primary murine group 2 innate lymphoid cells: a robust step-by-step approach | ||
FEBS Open Bio Metformin promotes mitochondrial integrity through AMPK-signaling in Leber's hereditary optic neuropathy. | ||
J Sci Food Agric Supplementation of epigallocatechin gallate in diet promotes growth performance, antioxidant capacity, and meat quality in growing-finishing pigs. |
