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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PGC1a Monoclonal antibody

PGC1a Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C1B2

Cat no : CL488-66369

Synonyms

LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PGC1a Monoclonal ant (CL488-66369)

    IF Staining of HeLa using CL488-66369

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PGC1a antibody (CL488-66369, Clone: 1C1B2 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PGC1a Monoclonal ant (CL488-66369)

    FC experiment of HeLa using CL488-66369

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human PGC1a (CL488-66369, Clone:1C1B2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-66369 targets PGC1a in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Calculated molecular weight 91 kDa
GenBank accession numberNM_013261
Gene symbol PPARGC1A
Gene ID (NCBI) 10891
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists a isoforms in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PGC1a antibody CL488-66369Download protocol
FC protocol for CL Plus 488 PGC1a antibody CL488-66369Download protocol
Standard Protocols
Click here to view our Standard Protocols