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4EBP1 Antibody 8 Publications

Mouse Monoclonal| Catalog number: 60246-1-Ig |CloneNo.: 2C3F3

Featured Product KD/KO validated

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Con: 240 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

4EBP1 Antibody WB K562 60246-1-Ig

WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells.

4EBP1 Antibody WB K-562 cells 60246-1-Ig

K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig(4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

4EBP1 Antibody IHC human pancreas tissue 60246-1-Ig

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 60246-1-Ig( 4EBP1 Antibody) at dilution of 1:400 (under 40x lens).

4EBP1 Antibody IF HeLa cells 60246-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells, K562 , RAW 264.7 cells
Positive IHC detected in:
human pancreas tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
4EBP1 fusion protein Ag18985
Full name:
eukaryotic translation initiation factor 4E binding protein 1
Calculated molecular weight:
118aa,12 kDa
Observed molecular weight:
15-24 kDa
GenBank accession number:
BC004459
Gene ID (NCBI):
1978
Gene symbol
EIF4EBP1
Synonyms
4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

Background

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

4EBP1 Mouse Monoclonal antibody

Yifei (Verified Customer), 11-01-2017
Applications
Western Blot
No image submitted with review

No image submitted with review

Lot Number
10004043
It works well for Western blot

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