|Positive WB detected in||K-562 cells, RAW 264.7 cells, NCI-H1299 cells, HCT 116 cells, C6 cells, K562|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60246-1-Ig is 4EBP1 Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||4EBP1 fusion protein Ag18985|
|Full Name||eukaryotic translation initiation factor 4E binding protein 1|
|Calculated molecular weight||118 aa, 12 kDa|
|Observed molecular weight||15-24 kDa|
|GenBank accession number||BC004459|
|Gene ID (NCBI)||1978|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (03-02-2021)
10ug of HEK293 lysate resolved on 4-20% gradient acrylamide gel. Membrane blocked (5% BSA) then incubated overnight with antibody (1:2,000). Incubated with secondary HRP antibody for visulization.
Yifei (Verified Customer) (11-01-2017)
It works well for Western blot