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4EBP1 Monoclonal antibody

4EBP1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C3F3

Cat no : 60246-1-Ig

Synonyms

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I



Tested Applications

Positive WB detected inK-562 cells, RAW 264.7 cells, NCI-H1299 cells, HCT 116 cells, C6 cells, K562
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 13 publications below

Product Information

The immunogen of 60246-1-Ig is 4EBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen 4EBP1 fusion protein Ag18985
Full Name eukaryotic translation initiation factor 4E binding protein 1
Calculated molecular weight 118 aa, 12 kDa
Observed molecular weight 15-24 kDa
GenBank accession numberBC004459
Gene symbol EIF4EBP1
Gene ID (NCBI) 1978
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)

Protocols

Product Specific Protocols
WB protocol for 4EBP1 antibody 60246-1-IgDownload protocol
IF protocol for 4EBP1 antibody 60246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Tissue Res

Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears.

Authors - Rongzong Zheng
mouseWB

FASEB J

Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling.

Authors - Yongxu Yang
humanWB

Signal Transduct Target Ther

A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo.

Authors - Yarui Ma
humanWB

Gene

MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer.

Authors - Hai-Feng Wang
humanWB

Oncol Rep

PMID: 28534965

Authors - Xuewen Liu
humanWB

Oncol Rep

EPS8 regulates proliferation, apoptosis and chemosensitivity in BCR-ABL positive cells via the BCR-ABL/PI3K/AKT/mTOR pathway.

Authors - Rui Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yifei (Verified Customer) (11-01-2017)

It works well for Western blot

  • Applications: Western Blot