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4EBP1 Monoclonal antibody
4EBP1 Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
2C3F3
Cat no : 60246-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, RAW 264.7 cells, NCI-H1299 cells, HCT 116 cells, C6 cells, K562 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 16 publications below |
IHC | See 1 publications below |
Product Information
The immunogen of 60246-1-Ig is 4EBP1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | 4EBP1 fusion protein Ag18985 |
Full Name | eukaryotic translation initiation factor 4E binding protein 1 |
Calculated molecular weight | 118 aa, 12 kDa |
Observed molecular weight | 15-24 kDa |
GenBank accession number | BC004459 |
Gene symbol | EIF4EBP1 |
Gene ID (NCBI) | 1978 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for 4EBP1 antibody 60246-1-Ig | Download protocol |
IF protocol for 4EBP1 antibody 60246-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Tissue Res Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears. | ||
FASEB J Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling. | ||
Signal Transduct Target Ther A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo. | ||
Aging (Albany NY) Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors. | ||
Gene MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yifei (Verified Customer) (11-01-2017) | It works well for Western blot
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