FcZero logo for Proteintech's Fc Silenced Recombinant Monoclonal Antibodies for Flow Cytometry

Eliminate background due to Fc receptor binding – without the need for Fc Receptor Block

Proteintech has designed recombinant antibodies featuring our all-new FcZero™ backbone engineered to silence the Fc domain for the cleanest flow cytometry data yet.

Part of the Uni-rAb™ family

Zero Fc receptor binding

Recombinant antibodies with Proteintech’s new FcZero backbone eliminates the need for Fc receptor block and simplifies your protocol.

Site-specific conjugation

FcZero-rAb features AccuBright™ site-specific, stoichiometric conjugation for precise control of degree of labeling, increased brightness, and removes the risk of fluorophores blocking the antigen-binding region.

One universal isotype

Simplify your protocol. FcZero-rAb recombinant antibodies use a Rabbit IgG backbone so that only one isotype control is needed.

Part of the Uni-rAb family

Uni-rAb recombinant monoclonal antibodies are developed using Proteintech’s Antigen-Specific B-Cell Cloning & Engineering (ABCE™) Platform for optimized specificity, affinity, purity, and consistency.

Comparison between conventional monoclonal antibodies for flow and recombinant antibodies for flow cytometry.
FcZero logo for Proteintech's Fc Silenced Recombinant Monoclonal Antibodies for Flow Cytometry

Non-specific binding of antibodies to Fc receptors in your sample can cause background signal in your data. The FcZero-rAb backbone features a mutation in the Fc receptor binding region to eliminate Fc receptor binding. The resulting data is cleaner than using a conventional antibody and Fc receptor block.

AccuBright™ Site-specific Conjugation

The FcZero recombinant antibody backbone features AccuBright site-specific conjugation technology, which allows us to precisely control the location of fluorophores on each IgG backbone. This ensures that fluorophores do not block the antigen-binding region of the antibody. Compared to traditional chemical conjugation methods, AccuBright also allows for precise control over the degree of labeling (DOL), or number of fluorophores conjugated to each antibody, so that signal intensity is consistent from lot to lot for ultimate dependability.

Gold standard clones with FCZero
Gold Standard Clone variable region with FcZero backbone

Gold-Standard Flow Cytometry Clones with FcZero™

Select gold standard clones for flow cytometry are now available with an FcZero-rAb™ backbone. The antigen-binding regions of these classic clones are combined with a Mouse IgG2a backbone that includes the FcZero mutation to the Fc receptor binding region and AccuBright™ site-specific conjugation. These antibodies have the top-cited and well-established specificity you’re used to with higher purity and zero non-specific background from Fc receptor binding.

Part of the Uni-rAb™ family

FcZero-rAb have all the advantages of our Uni-rAb recombinant antibodies, including:

  • Excellent specificity and high affinity
  • High throughput production
  • Unparalleled lot-to-lot consistency
  • High purity
  • Available in carrier-free formulations
Western blot analysis of various cell lysates using three separate batches of Uni-rAb™ Beta Actin Recombinant Antibody (81115-1-RR).

Western blot analysis of various cell lysates using three separate batches of Uni-rAb™ Beta Actin Recombinant Antibody (81115-1-RR).