|Positive WB detected in||Recombinant protein|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
10001-0-AP targets 6*his,His tag in WB, IF,ELISA applications and shows reactivity with recombinant protein samples.
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
Crystal structure of Human ASB9-2 and substrate-recognition of CKB.
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Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein.
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The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori.
Codon optimization, expression in Escherichia coli, and immunogenicity analysis of deformed wing virus (DWV) structural protein.
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