6*his,His tag Polyclonal antibody
6*his,His tag Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (1)
Applications
WB, IF,ELISA
Conjugate
Unconjugated
Cat no : 10001-0-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 10 publications below |
IF | See 1 publications below |
Product Information
10001-0-AP targets 6*his,His tag in WB, IF,ELISA applications and shows reactivity with recombinant protein samples.
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
Publications
Species | Application | Title |
---|---|---|
J Vet Diagn Invest Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein. | ||
Arch Insect Biochem Physiol The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori. | ||
PeerJ Codon optimization, expression in Escherichia coli, and immunogenicity analysis of deformed wing virus (DWV) structural protein. | ||
Virus Res Identification of a novel host protein interacting with the structural protein VP2 of deformed wing virus by yeast two-hybrid screening. | ||
Onco Targets Ther Fibroblast Activation Protein (FAP) Overexpression Induces Epithelial-Mesenchymal Transition (EMT) in Oral Squamous Cell Carcinoma by Down-Regulating Dipeptidyl Peptidase 9 (DPP9). |