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6*his,His tag Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 10001-0-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, ELISA
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6*his,His tag Antibody WB recombinant protein protein 10001-0-AP

Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (10001-0-AP) at various dilutions.

Species specificity:
recombinant protein, human
Positive WB detected in:
Recombinant protein
Recommended dilution:
WB : 1:500-1:2000
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Recombinant Protein
Full name:
6*his,His tag
Calculated molecular weight:
1 kDa
Observed molecular weight:
GenBank accession number:
Gene ID (NCBI):
Gene symbol
Synonyms
6 his, 6*his,His tag, his tag

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.


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