AARS2 Polyclonal antibody

AARS2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22696-1-AP

Synonyms

AARS2, AARSL, Alanine tRNA ligase, Alanyl tRNA synthetase like, AlaRS, KIAA1270, MT ALARS, MTALARS



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22696-1-AP is AARS2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AARS2 fusion protein Ag18539
Full Name alanyl-tRNA synthetase 2, mitochondrial (putative)
Calculated molecular weight 985 aa, 107 kDa
Observed molecular weight 105-115 kDa
GenBank accession numberBC131728
Gene symbol AARS2
Gene ID (NCBI) 57505
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.

Protocols

Product Specific Protocols
WB protocol for AARS2 antibody 22696-1-APDownload protocol
IHC protocol for AARS2 antibody 22696-1-APDownload protocol
IP protocol for AARS2 antibody 22696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols