AATF Polyclonal antibody

AATF Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10282-1-AP

Synonyms

AATF, CHE 1, CHE1, DED, Protein AATF, Rb binding protein Che 1, Traube



Tested Applications

Positive WB detected inHEK-293T cells, mouse brain tissue
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10282-1-AP targets AATF in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AATF fusion protein Ag0195
Full Name apoptosis antagonizing transcription factor
Calculated molecular weight 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC000591
Gene symbol AATF
Gene ID (NCBI) 26574
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.

Protocols

Product Specific Protocols
WB protocol for AATF antibody 10282-1-APDownload protocol
IF protocol for AATF antibody 10282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols