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ABHD5 Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 12201-1-AP

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ABHD5 Antibody WB Jurkat cells 12201-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 12201-1-AP(ABHD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ABHD5 Antibody IHC human skeletal muscle tissue 12201-1-AP

Immunohistochemical of paraffin-embedded human skeletal muscle using 12201-1-AP(ABHD5 antibody) at dilution of 1:50 (under 10x lens)

ABHD5 Antibody IHC human skeletal muscle tissue 12201-1-AP

Immunohistochemical of paraffin-embedded human skeletal muscle using 12201-1-AP(ABHD5 antibody) at dilution of 1:50 (under 40x lens)

ABHD5 Antibody IF MCF-7 cells 12201-1-AP

Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody 12201-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ABHD5 Antibody IP Jurkat cells 12201-1-AP

IP Result of anti-ABHD5 (IP:12201-1-AP, 4ug; Detection:12201-1-AP 1:500) with Jurkat cells lysate 4000ug.

Species specificity:
human, mouse, swine
Positive WB detected in:
Jurkat cells, human brain tissue, human cerebellum tissue, mouse heart tissue
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human skeletal muscle tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ABHD5 fusion protein Ag2840
Full name:
abhydrolase domain containing 5
Calculated molecular weight:
349aa,39 kDa
Observed molecular weight:
39 kDa
GenBank accession number:
BC021958
Gene ID (NCBI):
51099
Gene symbol
ABHD5
Synonyms
ABHD5, CDS, CGI58, IECN2, NCIE2

Background

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).


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