|Positive WB detected in||Jurkat cells, human brain tissue, human cerebellum tissue, mouse heart tissue|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human skeletal muscle tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12201-1-AP targets ABHD5 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ABHD5 fusion protein Ag2840|
|Full Name||abhydrolase domain containing 5|
|Calculated molecular weight||349 aa, 39 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC021958|
|Gene ID (NCBI)||51099|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).
|Product Specific Protocols|
|WB protocol for ABHD5 antibody 12201-1-AP||Download protocol|
|IHC protocol for ABHD5 antibody 12201-1-AP||Download protocol|
|IF protocol for ABHD5 antibody 12201-1-AP||Download protocol|
|IP protocol for ABHD5 antibody 12201-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Obes (Lond)
Maternal obesity aggravates the abnormality of porcine placenta by increasing N6-methyladenosine.
Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes.
Regulation of G0/G1 Switch Gene 2 (G0S2) Protein Ubiquitination and Stability by Triglyceride Accumulation and ATGL Interaction.
J Biol Chem
ABHD5 suppresses cancer cell anabolism through lipolysis-dependent activation of the AMPK/mTORC1 pathway.
J Lipid Res
CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development.
Am J Physiol Endocrinol Metab
Regulation of FSP27 protein stability by AMPK and HSC70.