Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of MCF-7 using 16215-1-AP
WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells.
WB analysis using 16215-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 16215-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16215-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 16215-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 16215-1-AP
IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug.
IHC staining of human liver using 16215-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 16215-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16215-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon using 16215-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 16215-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of HeLa using 16215-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ACAT1 Polyclonal antibody

Name: ACAT1 Polyclonal antibody
Brand: Proteintech
SKU: 16215-1-AP

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16215-1-AP

Publications(37)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (37)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human colon, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 32 publications below
IHC	See 6 publications below
IF	See 5 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ACAT1 fusion protein Ag9215 Predict reactive species
Full Name	acetyl-Coenzyme A acetyltransferase 1
Calculated Molecular Weight	45 kDa
Observed Molecular Weight	38-45 kDa
GenBank Accession Number	BC010942
Gene Symbol	ACAT1
Gene ID (NCBI)	38
RRID	AB_2220210
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P24752
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

Protocols

Product Specific Protocols
WB protocol for ACAT1 antibody 16215-1-AP	Download protocol
IHC protocol for ACAT1 antibody 16215-1-AP	Download protocol
IF protocol for ACAT1 antibody 16215-1-AP	Download protocol
IP protocol for ACAT1 antibody 16215-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration Authors - Zhuo Li View Article
mouse	WB	Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. Authors - Dong Huang View Article
human	WB	Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. Authors - De Huang View Article
Mouse	WB	J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. Authors - Shanshan Zhong View Article
mouse	WB	Sci Adv Ketogenic diet uncovers differential metabolic plasticity of brain cells Authors - Tim Düking View Article
mouse,human	WB	Phytomedicine Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux Authors - Jianfei Mu View Article