|Positive WB detected in||mouse heart tissue, rat skeletal muscle tissue, human skeletal muscle tissue, MCF-7 cells|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16215-1-AP targets ACAT1 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACAT1 fusion protein Ag9215|
|Full Name||acetyl-Coenzyme A acetyltransferase 1|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||38-45 kDa|
|GenBank accession number||BC010942|
|Gene ID (NCBI)||38|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.
|Product Specific Protocols|
|WB protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IHC protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IF protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IP protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|Click here to view our Standard Protocols|
TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth.
ACAT1 and Metabolism-Related Pathways Are Essential for the Progression of Clear Cell Renal Cell Carcinoma (ccRCC), as Determined by Co-expression Network Analysis.
Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
2-Deoxy-D-glucose treatment induces ketogenesis, sustains mitochondrial function, and reduces pathology in female mouse model of Alzheimer's disease.
J Cell Mol Med
Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1.