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- KD/KO Validated
ACAT1 Polyclonal antibody
ACAT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16215-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, rat skeletal muscle tissue, human skeletal muscle tissue, MCF-7 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
16215-1-AP targets ACAT1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACAT1 fusion protein Ag9215 |
| Full Name | acetyl-Coenzyme A acetyltransferase 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 38-45 kDa |
| GenBank accession number | BC010942 |
| Gene symbol | ACAT1 |
| Gene ID (NCBI) | 38 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACAT1 and ACAT2 are intracellular enzymes that convert free cholesterol to cholesteryl esters and play important roles in lipid metabolism. ACAT1 is widely expressed while ACAT2 is primarily expressed in intestine and liver. As the major ACAT isozyme in macrophages, ACTA1 plays an key role in foam cell formation and atherosclerosis, making it an appealing drug target for treating cardiovascular diseases. Recently it has been reported that ACTA1 is commonly activated by upregulated tetramerization in human cancers and is a promising anti-cancer target.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| IHC protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| IF protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| IP protocol for ACAT1 antibody 16215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes Dev TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth. | ||
Front Oncol ACAT1 and Metabolism-Related Pathways Are Essential for the Progression of Clear Cell Renal Cell Carcinoma (ccRCC), as Determined by Co-expression Network Analysis. | ||
Biomolecules Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite. | ||
Nat Metab Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis. | ||
Arch Med Sci Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages. | ||
Molecules Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells. |
