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  • KD/KO Validated

AFG3L2 Polyclonal antibody

AFG3L2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 14631-1-AP


AFG3 like protein 2, AFG3L2, Paraplegin like protein

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse brain tissue, mouse kidney tissue, mouse heart tissue, mouse skeletal muscle tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14631-1-AP targets AFG3L2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFG3L2 fusion protein Ag6209
Full Name AFG3 ATPase family gene 3-like 2 (yeast)
Calculated molecular weight 88 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC065016
Gene symbol AFG3L2
Gene ID (NCBI) 10939
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).


Product Specific Protocols
WB protocol for AFG3L2 antibody 14631-1-APDownload protocol
IHC protocol for AFG3L2 antibody 14631-1-APDownload protocol
IF protocol for AFG3L2 antibody 14631-1-APDownload protocol
IP protocol for AFG3L2 antibody 14631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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