Tested Applications
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, mouse kidney tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14631-1-AP targets AFG3L2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6209 Product name: Recombinant human AFG3L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 449-797 aa of BC065016 Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN Predict reactive species |
| Full Name | AFG3 ATPase family gene 3-like 2 (yeast) |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 80-90 kDa |
| GenBank Accession Number | BC065016 |
| Gene Symbol | AFG3L2 |
| Gene ID (NCBI) | 10939 |
| RRID | AB_2242420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4W6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
| IHC protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
| IP protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
| WB protocol for AFG3L2 antibody 14631-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
AFG3L2 Polyclonal antibody (Cat# 14631-1-AP) has 42 citations published across 28 journals, including Nature Cell Biology, Nature Communications, Molecular Cell, Science Advances, EMBO Journal, PNAS, and Cell Reports. Research fields span mitochondrial biology, protein quality control, mitophagy, ferroptosis, cancer, neurodegeneration, and metabolic regulation.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol A transport-independent role for SLC25A12 in mitochondrial stress signalling | ||
Cancer Res Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition | ||
Autophagy Mitochondrial dynamics and quality control regulate proteostasis in neuronal ischemia-reperfusion | ||
Nat Commun FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes | ||
Mol Cell The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism
|
Reviews
What customers say
The product has received one review from Boyan Zhang (April 2023), who rated it 5 stars. The reviewer used it for Western Blot and reported a clean band at the expected size. Overall, the limited feedback is positive, indicating reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (04-28-2023) | clean band around the expected size
|
