AGPAT6 Polyclonal antibody

AGPAT6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16762-1-AP

Synonyms

1 AGP acyltransferase 6, 1 AGPAT 6, AGPAT6, GPAT4, LPAAT zeta, LPAATZ, TSARG7



Tested Applications

Positive WB detected inmouse testis tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16762-1-AP targets AGPAT6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGPAT6 fusion protein Ag10294
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta)
Calculated molecular weight 456 aa, 52 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC051377
Gene symbol AGPAT6
Gene ID (NCBI) 137964
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AGPAT6 antibody 16762-1-APDownload protocol
IHC protocol for AGPAT6 antibody 16762-1-APDownload protocol
IP protocol for AGPAT6 antibody 16762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Invest Ophthalmol Vis Sci

Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.

Authors - Sasha M Milbeck