AGTR1 Antibody

AGTR1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 25343-1-AP

Synonyms

AG2S, AGTR1, AGTR1A, AGTR1B, Angiotensin II type 1 receptor, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, AT2R1A, AT2R1B, HAT1R, Type 1 angiotensin II receptor



Tested Applications

Positive WB detected inC2C12 cells, PC-12 cells, mouse kidney tissue, mouse heart tissue, HuH-7 cells, HEK-293 cells
Positive IHC detected inhuman heart tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 25343-1-AP immunogen is Fusion Protein AGTR1 protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGTR1 fusion protein Ag14461
Full Name angiotensin II receptor, type 1
Calculated molecular weight 359aa,41 kDa
Observed molecular weight 50 kDa
GenBank accession number BC022447
Gene symbol AGTR1
Gene ID (NCBI) 185
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named as AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Protocols

Product Specific Protocols
WB protocol for AGTR1 antibody 25343-1-APDownload protocol
IHC protocol for AGTR1 antibody 25343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Am J Chin Med

Puerarin Reduces Blood Pressure in Spontaneously Hypertensive Rats by Targeting eNOS.

Authors - Weili Shi
mouseIHC

Ecotoxicol Environ Saf

Comparing the effects of diethylhexyl phthalate and dibutyl phthalate exposure on hypertension in mice.

Authors - Xiaoman Xie
WB

J Cell Physiol

Knockdown of periostin attenuates 5/6 nephrectomy-induced intrarenal renin-angiotensin system activation, fibrosis, and inflammation in rats.

Authors - Xiaohui Bian
ratIHC

J Cell Mol Med

Therapeutic effects of higenamine combined with [6]-gingerol on chronic heart failure induced by doxorubicin via ameliorating mitochondrial function.

Authors - Jianxia Wen
ratWB

Neural Plast

Electroacupuncture Improved Chronic Cerebral Hypoperfusion-Induced Anxiety-Like Behavior and Memory Impairments in Spontaneously Hypertensive Rats by Downregulating the ACE/Ang II/AT1R Axis and Upregulating the ACE2/Ang-(1-7)/MasR Axis.

Authors - Peipei Feng
humanWB

Biomed Res Int

PBX3 Promotes Tumor Growth and Angiogenesis via Activation of AT1R/VEGFR2 Pathway in Papillary Thyroid Carcinoma.

Authors - Qin Chen