Filter: AllWBIHC

WB analysis using 25343-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 25343-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 25343-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 25343-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 25343-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25343-1-AP (AGTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse heart using 25343-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 25343-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25343-1-AP (AGTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 25343-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 25343-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 25343-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25343-1-AP (AGTR1 Antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

AGTR1 Polyclonal antibody

AGTR1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25343-1-AP

Publications(68)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

AT1, Angiotensin II type-1 receptor, Angiotensin II type 1 receptor, AGTR1B, AGTR1A

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (68)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	C2C12 cells, PC-12 cells, mouse kidney tissue, mouse heart tissue, HuH-7 cells, HEK-293 cells
Positive IHC detected in	mouse heart tissue, human liver cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 55 publications below
IHC	See 16 publications below
IF	See 14 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

25343-1-AP targets AGTR1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AGTR1 fusion protein Ag14461 Predict reactive species
Full Name	angiotensin II receptor, type 1
Calculated Molecular Weight	359 aa, 41 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC022447
Gene Symbol	AGTR1
Gene ID (NCBI)	185
RRID	AB_2880037
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P30556
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, and salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Protocols

Product Specific Protocols
WB protocol for AGTR1 antibody 25343-1-AP	Download protocol
IHC protocol for AGTR1 antibody 25343-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,CoIP	Cell Res Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury. Authors - Yi Fu View Article
human	WB	Adv Sci (Weinh) Transcriptomics-Based Liquid Biopsy for Early Detection of Recurrence in Locally Advanced Gastric Cancer Authors - Ping'an Ding KD Validated View Article
mouse	WB	Aging Cell Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling Authors - Ruili Dang View Article
human	IF	Biomaterials Inhibition of tumor-promoting stroma to enforce subsequently targeting AT1R on tumor cells by pathological inspired micelles. Authors - Yun Zhu View Article
mouse	WB	Redox Biol Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system. Authors - Changmeng Cui View Article
mouse,rat	WB,IF	Free Radic Biol Med Spinal AT1R contributes to neuroinflammation and neuropathic pain via NOX2-dependent redox signaling in microglia Authors - Wencui Zhang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Kazu (Verified Customer) (12-14-2022)	This antibody worked well on mouse optic nerve sections at optimized dilution of 1:100. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: Optic nerve
FH Wu (Verified Customer) (07-16-2021)	Works good for IHC assay Applications: Immunohistochemistry Cell Tissue Type: glandular lumen AGTR1 Antibody Immunohistochemistry validation ( dilution) in glandular lumen (Cat no:25343-1-AP)