Fetuin-A Polyclonal antibody

Fetuin-A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16571-1-AP

Synonyms

A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, Fetuin-A, HSGA



Tested Applications

Positive WB detected inhuman liver tissue, human heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16571-1-AP targets Fetuin-A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity Plasmodium falciparum
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fetuin-A fusion protein Ag9853
Full Name alpha-2-HS-glycoprotein
Calculated molecular weight 367 aa, 39 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC048198
Gene symbol AHSG
Gene ID (NCBI) 197
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fetuin-A, also named as Alpha2-HS glycoprotein (AHSG), is a member of cystatin superfamily of protease inhibitors. It is a highly expressed glycoprotein in various fetal tissues whereas it is mainly expressed by the liver in adults. Fetuin A, an extracellular inhibitor of transforming growth factor β, is a profibrogenic stimulus in liver disease. Circulating fetuin-A may be a beneficial serum biomarker in the detection of liver and vascular fibrosis progression in patients with non-alcoholic fatty liver disease. It is also involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuin-A exerts its effects on the cardiovascular system by two different mechanisms. On the one hand it inhibits ins signaling and induces ins resistance contributing to the onset of atherosclerosis and on the other hand it inhibits calcium deposition and protects from vascular calcification.

Protocols

Product Specific Protocols
WB protocol for Fetuin-A antibody 16571-1-APDownload protocol
IHC protocol for Fetuin-A antibody 16571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Plasmodium falciparumWB

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan