|Positive WB detected in||K-562 cells, A549 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse testis tissue, rat testis tissue, SH-SY5Y cells|
|Positive IHC detected in||human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The 20590-1-AP immunogen is Fusion Protein AIM2 protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AIM2 fusion protein Ag14520|
|Full Name||absent in melanoma 2|
|Calculated molecular weight||343aa,39 kDa|
|Observed molecular weight||39-45 kDa|
|GenBank accession number||BC010940|
|Gene ID (NCBI)||9447|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831].
Endoplasmic Reticulum Stress Induces Macrophages to Produce IL-1β During Mycobacterium bovis Infection via a Positive Feedback Loop Between Mitochondrial Damage and Inflammasome Activation.
Neural Regen Res
Expression and localization of absent in melanoma 2 in the injured spinal cord.
Correlation of AIM2 expression in peripheral blood mononuclear cells from humans with acute and chronic hepatitis B.
Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.
AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection.