AIM2 Antibody

AIM2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20590-1-AP

Synonyms

absent in melanoma 2, AIM2, PYHIN4



Tested Applications

Positive WB detected inK-562 cells, A549 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse testis tissue, rat testis tissue, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 20590-1-AP immunogen is Fusion Protein AIM2 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14520
Full Name absent in melanoma 2
Calculated molecular weight 343aa,39 kDa
Observed molecular weight 39-45 kDa
GenBank accession number BC010940
Gene symbol AIM2
Gene ID (NCBI) 9447
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831].

Protocols

Product Specific Protocols
WB protocol for AIM2 antibody 20590-1-APDownload protocol
IHC protocol for AIM2 antibody 20590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Endoplasmic Reticulum Stress Induces Macrophages to Produce IL-1β During Mycobacterium bovis Infection via a Positive Feedback Loop Between Mitochondrial Damage and Inflammasome Activation.

Authors - Yi Liao
ratWB,IF

Neural Regen Res

Expression and localization of absent in melanoma 2 in the injured spinal cord.

Authors - Sai-Nan Wang
humanWB

Hum Immunol

Correlation of AIM2 expression in peripheral blood mononuclear cells from humans with acute and chronic hepatitis B.

Authors - Wu Dian-Lei DL
humanIHC,IF

Front Immunol

Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.

Authors - Qingling Guo
mouseWB

Mucosal Immunol

AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection.

Authors - G-Q Hu