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AK3 Polyclonal antibody

AK3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12562-1-AP

Synonyms

adenylate kinase 3, AK 3, AK3, AK3L1, AK6, AKL3L, AKL3L1, FIX

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of human placenta using 12562-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of BxPC-3 using 12562-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of mouse kidney using 12562-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of L02 using 12562-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of L02 using 12562-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of MCF-7 using 12562-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of MCF-7 using 12562-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of HepG2 using 12562-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of HepG2 using 12562-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of mouse heart using 12562-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of mouse heart using 12562-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of mouse kidney using 12562-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of HEK-293 using 12562-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of HEK-293 using 12562-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of human placenta using 12562-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of human placenta using 12562-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of mouse kidney using 12562-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of mouse kidney using 12562-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of BxPC-3 using 12562-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of BxPC-3 using 12562-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of human kidney using 12562-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using AK3 Polyclonal antibody (12562-1-AP)

    WB analysis of Y79 using 12562-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using AK3 Polyclonal antibody (12562-1-AP)

    IP experiment of mouse kidney using 12562-1-AP

    IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using AK3 Polyclonal antibody (12562-1-AP)

    IHC staining of human pancreas cancer using 12562-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AK3 Polyclonal antibody (12562-1-AP)

    IHC staining of human heart using 12562-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AK3 Polyclonal antibody (12562-1-AP)

    IHC staining of human heart using 12562-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AK3 Polyclonal antibody (12562-1-AP)

    IF Staining of HepG2 using 12562-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12562-1-AP (AK3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue, BxPC-3 cells, HEK-293 cells, HepG2 cells, human kidney tissue, L02 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, Y79 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman pancreas cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12562-1-AP targets AK3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AK3 fusion protein Ag3146
Full Name adenylate kinase 3
Calculated Molecular Weight 227 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC013771
Gene Symbol AK3
Gene ID (NCBI) 50808
RRIDAB_2305373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for AK3 antibody 12562-1-APDownload protocol
IHC protocol for AK3 antibody 12562-1-APDownload protocol
IF protocol for AK3 antibody 12562-1-APDownload protocol
IP protocol for AK3 antibody 12562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

MicroRNA‑214 upregulates HIF‑1α and VEGF by targeting ING4 in lung cancer cells.

Authors - Yue Li
View Article
humanWB

J Gene Med

MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.

Authors - Wei-Yan Qin
View Article