|Positive WB detected in||rat brain tissue|
|Positive IHC detected in||mouse brain tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25199-1-AP is AKAP12 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AKAP12 fusion protein Ag17769|
|Full Name||A kinase (PRKA) anchor protein 12|
|Calculated molecular weight||1782 aa, 191 kDa|
|Observed molecular weight||200 kDa|
|GenBank accession number||BC157067|
|Gene ID (NCBI)||9590|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Pradeep (Verified Customer) (07-20-2020)
I used for immunofluorescence in mouse tissue sample. it is working great.
Rushita (Verified Customer) (11-26-2019)
The Ab works very well for detection of endogenous AKAP12 for western blotting (1:1000) in HEK 293 and COS7 cell lines.