AKAP12 Polyclonal antibody

AKAP12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25199-1-AP

Synonyms

A kinase anchor protein 12, AKAP 12, AKAP 250, AKAP12, AKAP250, Gravin, Myasthenia gravis autoantigen



Tested Applications

Positive WB detected inrat brain tissue
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25199-1-AP is AKAP12 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP12 fusion protein Ag17769
Full Name A kinase (PRKA) anchor protein 12
Calculated molecular weight 1782 aa, 191 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC157067
Gene symbol AKAP12
Gene ID (NCBI) 9590
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).

Protocols

Product Specific Protocols
WB protocol for AKAP12 antibody 25199-1-APDownload protocol
IHC protocol for AKAP12 antibody 25199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Systematic analysis of mRNA expression profiles in NSCLC cell lines to screen metastasis-related genes.

Authors - Ying Liu
humanWB

Am J Transl Res

Comprehensive analysis of differentially expressed non-coding RNAs and mRNAs in gastric cancer cells under hypoxic conditions.

Authors - Jia Li
mouseIF

Sci Rep

β-catenin regulates the formation of multiple nephron segments in the mouse kidney.

Authors - Patrick Deacon
humanWB

Mol Ther Nucleic Acids

The Human Novel Gene LNC-HC Inhibits Hepatocellular Carcinoma Cell Proliferation by Sequestering hsa-miR-183-5p.

Authors - Xi Lan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pradeep (Verified Customer) (07-20-2020)

I used for immunofluorescence in mouse tissue sample. it is working great.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse HLI
FH

Rushita (Verified Customer) (11-26-2019)

The Ab works very well for detection of endogenous AKAP12 for western blotting (1:1000) in HEK 293 and COS7 cell lines.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, COS7