|Positive WB detected in||human brain tissue, human heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12591-1-AP targets AKAP7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AKAP7 fusion protein Ag3297|
|Full Name||A kinase (PRKA) anchor protein 7|
|Calculated molecular weight||81 aa, 9 kDa|
|Observed molecular weight||11 kDa, 18 kDa, 37 kDa|
|GenBank accession number||BC016927|
|Gene ID (NCBI)||9465|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 15-18 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7.
|Product Specific Protocols|
|WB protocol for AKAP7 antibody 12591-1-AP||Download protocol|
|IHC protocol for AKAP7 antibody 12591-1-AP||Download protocol|
|IF protocol for AKAP7 antibody 12591-1-AP||Download protocol|
|IP protocol for AKAP7 antibody 12591-1-AP||Download protocol|
|Click here to view our Standard Protocols|
iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.
Murine AKAP7 Has a 2',5'-Phosphodiesterase Domain That Can Complement an Inactive Murine Coronavirus ns2 Gene.
Targeted deletion of AKAP7 in dentate granule cells impairs spatial discrimination.
Proc Natl Acad Sci U S A
Cardiomyocytes from AKAP7 knockout mice respond normally to adrenergic stimulation.