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  • KD/KO Validated

AKAP7 Antibody

AKAP7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12591-1-AP

Synonyms

A kinase anchor protein 18 kDa, AKAP 18, AKAP 7 isoforms alpha and beta, AKAP18, AKAP7



Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12591-1-AP targets AKAP7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP7 fusion protein Ag3297
Full Name A kinase (PRKA) anchor protein 7
Calculated molecular weight 81aa,9 kDa
Observed molecular weight 11 kDa,18 kDa,37 kDa
GenBank accession number BC016927
Gene symbol AKAP7
Gene ID (NCBI) 9465
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP7, also named as AKAP15 and AKAP18, localize PKA in complexes with CaV1.2 and PLN, respectively. AKAP7 targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. AKAP7 has short isoform AKAP7 alpha and AKAP7 beta with MW 15-18 kDa; long isoform AKAP7 gamma with MW 37-42 kDa. AKAP7alpha was highly expressed only in brain and weakly in lung lysates from WT animals. This antibody can recognize all the isoforms of AKAP7.

Protocols

Product Specific Protocols
WB protocol for AKAP7 antibody 12591-1-APDownload protocol
IHC protocol for AKAP7 antibody 12591-1-APDownload protocol
IF protocol for AKAP7 antibody 12591-1-APDownload protocol
IP protocol for AKAP7 antibody 12591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Proteomics

iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.

Authors - Hongmei Lu
human, mouseWB

MBio

Murine AKAP7 Has a 2',5'-Phosphodiesterase Domain That Can Complement an Inactive Murine Coronavirus ns2 Gene.

Authors - Elona Gusho
mouseWB,IF

Elife

Targeted deletion of AKAP7 in dentate granule cells impairs spatial discrimination.

Authors - Brian W Jones
  • KO Validated
mouseWB

Proc Natl Acad Sci U S A

Cardiomyocytes from AKAP7 knockout mice respond normally to adrenergic stimulation.

Authors - Jones Brian W BW
  • KO Validated