AKR1B1 Polyclonal antibody

AKR1B1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15439-1-AP

Synonyms

ADR, AKR1B1, Aldehyde reductase, Aldose reductase, ALDR1, ALR2, AR



Tested Applications

Positive WB detected inA431 cells, L02 cells, human liver tissue, BxPC-3 cells, HepG2 cells, human placenta tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

15439-1-AP targets AKR1B1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1B1 fusion protein Ag7142
Full Name aldo-keto reductase family 1, member B1 (aldose reductase)
Calculated molecular weight 36 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000260
Gene symbol AKR1B1
Gene ID (NCBI) 231
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AKR1B1 antibody 15439-1-APDownload protocol
IF protocol for AKR1B1 antibody 15439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Fertil Steril

Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation.

Authors - Valentina Murdica
ratWB

Oxid Med Cell Longev

WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.

Authors - Xiaoyu Zhou
humanWB

BMC Nephrol

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

Authors - Yang Lu
humanWB

Biochem Pharmacol

Regulation on SIRT1-PGC-1α/Nrf2 pathway together with selective inhibition of aldose reductase makes compound hr5F a potential agent for the treatment of diabetic complications.

Authors - Zhihua Wang