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AKT1 Antibody

AKT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51077-1-AP

Synonyms

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha



Tested Applications

Positive WB detected inmulti-cells, COLO 320 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

51077-1-AP targets AKT1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
Observed molecular weight 56-62 kDa
GenBank accession number BC084538
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
WB protocol for AKT1 antibody 51077-1-APDownload protocol
IHC protocol for AKT1 antibody 51077-1-APDownload protocol
IF protocol for AKT1 antibody 51077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Identification of aberrantly methylated differentially expressed genes in prostate carcinoma using integrated bioinformatics.

Authors - Kai Wu
humanWB

Biomark Med

Gelsolin-like actin-capping protein is associated with patient prognosis, cellular apoptosis and proliferation in prostate cancer.

Authors - Tieqiu Li
humanWB

Evid Based Complement Alternat Med

Comparative Effect of Aqueous and Methanolic Bupleuri Radix Extracts on Hepatic Uptake of High-Density Lipoprotein and Identification of the Potential Target in HFD-Fed Mice.

Authors - Zhibin Tan
humanWB

Autoimmunity

Clinical significance of combined detection of interleukin-6 and tumour markers in lung cancer.

Authors - Hexiao Tang
humanWB

Autoimmunity

Oestrogen receptor β5 and epidermal growth factor receptor synergistically promote lung cancer progression.

Authors - Yuquan Bai
humanWB

J Cell Biochem

Interaction between epidermal growth factor receptor and interleukin-6 receptor in NSCLC progression.

Authors - Bai Yuquan