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AKT1 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 51077-1-AP

Featured Product KD/KO validated

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Con: 28 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

AKT1 Antibody WB MCF-7 cells 51077-1-AP

WB result of AKT1 antibody (51077-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells

AKT1 Antibody WB multi-cells 51077-1-AP

WB result of anti-AKT1 (51077-1-AP) in different cell lysates.

AKT1 Antibody IHC human breast cancer tissue 51077-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 51077-1-AP(AKT1 antibody) at dilution of 1:50 (under 10x lens)

AKT1 Antibody IHC human breast cancer tissue 51077-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 51077-1-AP(AKT1 antibody) at dilution of 1:50 (under 40x lens)

AKT1 Antibody IF HeLa cells 51077-1-AP

Immunofluorescent analysis of HeLa cells using 51077-1-AP(AKT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human
Positive WB detected in:
multi-cells, COLO 320 cells, MCF-7 cells
Positive IHC detected in:
human breast cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight:
56 kDa
Observed molecular weight:
56-62 kDa
GenBank accession number:
BC084538
Gene ID (NCBI):
207
Gene symbol
AKT1
Synonyms
AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Background

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.


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