|Positive WB detected in||K-562 cells, HL-60 cells, mouse liver tissue, mouse brain tissue, mouse kidney tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human testis tissue, human liver tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse colon tissue, HepG2 cells, mouse testis tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15910-1-AP targets ALDH1A1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ALDH1A1 fusion protein Ag8665|
|Full Name||aldehyde dehydrogenase 1 family, member A1|
|Calculated molecular weight||501 aa, 55 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC001505|
|Gene ID (NCBI)||216|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.
|Product Specific Protocols|
|WB protocol for ALDH1A1 antibody 15910-1-AP||Download protocol|
|IHC protocol for ALDH1A1 antibody 15910-1-AP||Download protocol|
|IF protocol for ALDH1A1 antibody 15910-1-AP||Download protocol|
|IP protocol for ALDH1A1 antibody 15910-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Susmita (Verified Customer) (09-17-2020)
Antibody is working good for WB
Mario Armando (Verified Customer) (02-18-2020)
I used this ALDH1A1 antibody on different human and mouse tissues including adipose tissue, heart and skeletal muscle. It worked wonderfully even when using paraffin embedded sections in both human and mouse.
Joleen (Verified Customer) (06-07-2019)
Prominent band correspond to ALDH1A1. Works well in western blot.