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ALDH1A1 Antibody 35 Publications

Rabbit Polyclonal| Catalog number: 15910-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ALDH1A1 Antibody WB K-562 cells 15910-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

ALDH1A1 Antibody IHC human testis tissue 15910-1-AP

Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15910-1-AP( ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens).

ALDH1A1 Antibody IHC human testis tissue 15910-1-AP

Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15910-1-AP( ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens).

ALDH1A1 Antibody IP mouse liver tissue 15910-1-AP

IP Result of anti-ALDH1A1 (IP:15910-1-AP, 4ug; Detection:15910-1-AP 1:500) with mouse liver tissue lysate 6400ug.

ALDH1A1 Antibody IF mouse testis tissue 15910-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse testis tissue using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ALDH1A1 Antibody IF mouse testis tissue 15910-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse testis tissue using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ALDH1A1 Antibody IF HepG2 cells 15910-1-AP

Immunofluorescent analysis of HepG2 cells using 15910-1-AP(ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells, HL-60 cells, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human testis tissue, human eye tissue, human liver tissue, mouse testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, mouse testis tissue
Recommended dilution:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ALDH1A1 fusion protein Ag8665
Full name:
aldehyde dehydrogenase 1 family, member A1
Calculated molecular weight:
501aa,55 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC001505
Gene ID (NCBI):
216
Gene symbol
ALDH1A1
Synonyms
ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

Background

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.


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