ALDH1A2 Polyclonal antibody

ALDH1A2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13951-1-AP

Synonyms

ALDH1A2, RALDH 2, RALDH(II), RALDH2, RALDH2 T, Retinal dehydrogenase 2



Tested Applications

Positive WB detected inK-562 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13951-1-AP targets ALDH1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A2 fusion protein Ag5062
Full Name aldehyde dehydrogenase 1 family, member A2
Calculated molecular weight 518 aa, 57 kDa
Observed molecular weight 53-57 kDa
GenBank accession numberBC030589
Gene symbol ALDH1A2
Gene ID (NCBI) 8854
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ALDH1A2 antibody 13951-1-APDownload protocol
IHC protocol for ALDH1A2 antibody 13951-1-APDownload protocol
IP protocol for ALDH1A2 antibody 13951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Tracing Tumor Evolution in Sarcoma Reveals Clonal Origin of Advanced Metastasis.

Authors - Yuning J Tang
mouseIHC

Biol Reprod

Sources of All-Trans Retinal Oxidation Independent of the Aldehyde Dehydrogenase 1A Isozymes Exist in the Postnatal Testis.

Authors - My-Thanh Beedle
humanWB,IHC

Biol Reprod

ALDH Enzyme Expression Is Independent of the Spermatogenic Cycle and Their Inhibition Causes Misregulation of Murine Spermatogenic Processes.

Authors - Travis Kent
mouseWB

PLoS One

Retinoic acid signalling is activated in the postischemic heart and may influence remodelling.

Authors - Bilbija Dusan D
humanIHC

J Lipid Res

Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations.

Authors - Samuel L Arnold
humanWB

Elife

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

Authors - Carlos J Diaz Osterman