Amylase Alpha Monoclonal antibody

Amylase Alpha Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2F1G8

Cat no : 66133-1-Ig

Synonyms

AMY2A, Amylase, alpha, Pancreatic alpha amylase



Tested Applications

Positive WB detected inhuman saliva, tissue, pig pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 66133-1-Ig is Amylase Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Amylase Alpha fusion protein Ag8819
Full Name amylase, alpha 2A (pancreatic)
Calculated molecular weight 511 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC007060
Gene symbol AMY2A
Gene ID (NCBI) 279
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.

Protocols

Product Specific Protocols
WB protocol for Amylase Alpha antibody 66133-1-IgDownload protocol
IHC protocol for Amylase Alpha antibody 66133-1-IgDownload protocol
IF protocol for Amylase Alpha antibody 66133-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Toxicol Pathol

Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat.

Authors - Yusuke Kuroda