Filter: AllWBIPIHCIF/ICC

WB analysis using 18374-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 18374-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18374-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 18374-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 18374-1-AP
IP result of anti-ANGPTL4 (IP:18374-1-AP, 4ug; Detection:18374-1-AP 1:500) with mouse heart tissue lysate 4000ug.
IHC staining of human kidney using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.
IHC staining of human kidney using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.
IHC staining of human heart using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.
IHC staining of human heart using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.
IHC staining of human ovary using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.
IHC staining of human ovary using 18374-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary slide using 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:50.
IF Staining of NIH/3T3 using 18374-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HT-29 using 18374-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HEK-293 using 18374-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

ANGPTL4 Polyclonal antibody

Name: ANGPTL4 Polyclonal antibody
Brand: Proteintech
SKU: 18374-1-AP
Rating: 5.00 (1 reviews)

ANGPTL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18374-1-AP

Publications(45)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ANGPTL2, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (45)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, mouse kidney tissue, HEK-293 cells, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human kidney tissue, human oesophagus cancer tissue, human heart tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, HT-29 cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 28 publications below
IHC	See 18 publications below
IF	See 9 publications below
IP	See 1 publications below

Product Information

18374-1-AP targets ANGPTL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ANGPTL4 fusion protein Ag13237 Predict reactive species
Full Name	angiopoietin-like 4
Calculated Molecular Weight	45 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC023647
Gene Symbol	ANGPTL4
Gene ID (NCBI)	51129
RRID	AB_2878539
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BY76
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Protocols

Product Specific Protocols
WB protocol for ANGPTL4 antibody 18374-1-AP	Download protocol
IHC protocol for ANGPTL4 antibody 18374-1-AP	Download protocol
IF protocol for ANGPTL4 antibody 18374-1-AP	Download protocol
IP protocol for ANGPTL4 antibody 18374-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nat Metab Resistant starch intake facilitates weight loss in humans by reshaping the gut microbiota Authors - Huating Li View Article
mouse	IHC	J Hazard Mater Insight into perfluorooctanoic acid-induced impairment of mouse embryo implantation via single-cell RNA-seq Authors - Xiaoqiang Sheng View Article
human	IP	J Clin Invest Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema. Authors - Akrit Sodhi View Article
mouse	WB	EMBO J LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling. Authors - Yanying Wang View Article
mouse	IF	J Pathol ANGPTL4-αvβ3 integrin interaction counteracts hypoxia-induced vascular permeability by modulating Src signalling downstream of VEGFR2. Authors - Elisa Gomez Perdiguero View Article
human	IHC	Breast Cancer Res An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer. Authors - Misako Sato View Article