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ANGPTL4 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 18374-1-AP

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Con: 43 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

ANGPTL4 Antibody WB mouse kidney tissue 18374-1-AP

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP( ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

ANGPTL4 Antibody IHC human oesophagus cancer tissue 18374-1-AP

Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP( ANGPTL4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ANGPTL4 Antibody IHC human oesophagus cancer tissue 18374-1-AP

Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP( ANGPTL4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ANGPTL4 Antibody IP mouse heart tissue 18374-1-AP

IP Result of anti-ANGPTL4 (IP:18374-1-AP, 4ug; Detection:18374-1-AP 1:500) with mouse heart tissue lysate 4000ug.

ANGPTL4 Antibody IF NIH/3T3 cells 18374-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed NIH/3T3 cells using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
mouse kidney tissue, HEK-293 cells, mouse heart tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
human oesophagus cancer tissue, human heart tissue, human kidney tissue, human ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NIH/3T3 cells, HEK-293 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ANGPTL4 fusion protein Ag13237
Full name:
angiopoietin-like 4
Calculated molecular weight:
45 kDa
Observed molecular weight:
45-50 kDa
GenBank accession number:
BC023647
Gene ID (NCBI):
51129
Gene symbol
ANGPTL4
Synonyms
angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL2, ANGPTL4, ARP4, FIAF, HFARP, NL2, PGAR, pp1158, PSEC0166, UNQ171/PRO197

Background

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes.


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