ANGPTL4 Polyclonal antibody
ANGPTL4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18374-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, mouse heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human oesophagus cancer tissue, human heart tissue, human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 18374-1-AP is ANGPTL4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ANGPTL4 fusion protein Ag13237 |
| Full Name | angiopoietin-like 4 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC023647 |
| Gene symbol | ANGPTL4 |
| Gene ID (NCBI) | 51129 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANGPTL4 antibody 18374-1-AP | Download protocol |
| IHC protocol for ANGPTL4 antibody 18374-1-AP | Download protocol |
| IF protocol for ANGPTL4 antibody 18374-1-AP | Download protocol |
| IP protocol for ANGPTL4 antibody 18374-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Med Sci Monit Angiopoietin-Like Protein 4 (ANGPTL4) Induces Retinal Pigment Epithelial Barrier Breakdown by Activating Signal Transducer and Activator of Transcription 3 (STAT3): Evidence from ARPE-19 Cells Under Hypoxic Condition and Diabetic Rats. | ||
Oncol Rep Hypoxia-induced angiopoietin-like protein 4 as a clinical biomarker and treatment target for human prostate cancer. | ||
J Cell Mol Med The long non-coding RNA PVT1 represses ANGPTL4 transcription through binding with EZH2 in trophoblast cell. | ||
J Cell Mol Med Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure. | ||
Breast Cancer Res An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer. |
