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ANP32A Polyclonal antibody

ANP32A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 15810-1-AP

Synonyms

ANP32A, C15orf1, I1PP2A, LANP, MAPM, Mapmodulin, PHAP1, PHAPI, PP32



Tested Applications

Positive WB detected inA549 cells, human brain tissue, mouse brain tissue, mouse thymus tissue, Raji cells, HeLa cells, MCF-7 cells, HEK-293T cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, A2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15810-1-AP targets ANP32A in WB, IHC, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANP32A fusion protein Ag8546
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Calculated Molecular Weight 249 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC007200
Gene Symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2056302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Protocols

Product Specific Protocols
WB protocol for ANP32A antibody 15810-1-APDownload protocol
IHC protocol for ANP32A antibody 15810-1-APDownload protocol
IF protocol for ANP32A antibody 15810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arch Virol

The interaction of cellular protein ANP32A with influenza A virus polymerase component PB2 promotes vRNA synthesis.

Authors - Xiuli Wei
  • KD Validated