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  • KD/KO Validated

ANTXR2 Polyclonal antibody

ANTXR2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16723-1-AP

Synonyms

anthrax toxin receptor 2, ANTXR2, CMG 2, CMG2, ISH, JHF



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tissue, human kidney tissue, human placenta tissue, human testis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16723-1-AP targets ANTXR2 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, Camelus bactrianus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANTXR2 fusion protein Ag10162
Full Name anthrax toxin receptor 2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC107876
Gene Symbol ANTXR2
Gene ID (NCBI) 118429
RRIDAB_2056741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANTXR2 (anthrax toxin receptor 2), also known as CMG2 (capillary morphogenesis gene 2 protein) is a transmembrane protein that is induced during capillary morphogenesis. It contains a vWA domain that shows strong binding to laminin and collagen IV, suggesting that this protein plays a role in basement membrane matrix assembly and endothelial cell morphogenesis (PMID: 11683410; 14508707). Like its paralog TEM8 (ANTXR1), ANTXR2 also functions as a receptor for anthrax toxin. Mutations in ANTXR2 result in the allelic disorders juvenile hyaline fibromatosis and infantile systemic hyalinosis (PMID: 14508707; 12973667; 22383261).

Protocols

Product Specific Protocols
WB protocol for ANTXR2 antibody 16723-1-APDownload protocol
IHC protocol for ANTXR2 antibody 16723-1-APDownload protocol
IP protocol for ANTXR2 antibody 16723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Angiogenesis

Capillary morphogenesis gene 2 (CMG2) mediates growth factor-induced angiogenesis by regulating endothelial cell chemotaxis.

Authors - Lorna M Cryan
  • KO Validated
humanWB,IHC,CoIP

Oncogene

Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway.

Authors - Chengdong Ji
humanIHC

J Pathol

Capillary morphogenesis protein 2 is a novel prognostic biomarker and plays oncogenic roles in glioma.

Authors - Juan Tan
humanIHC

FASEB J

Genetic deletion of CMG2 exacerbates systemic-to-pulmonary shunt-induced pulmonary arterial hypertension.

Authors - Liukun Meng
Camelus bactrianusWB

Reprod Domest Anim

Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.

Authors - Qi Wang
humanWB,IP

Proc Natl Acad Sci U S A

Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes.

Authors - Oksana A Sergeeva