|Positive WB detected in||HEK-293 cells, human heart tissue, human spleen tissue, mouse spleen tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11401-1-AP targets Alpha Adaptin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha Adaptin fusion protein Ag1927|
|Full Name||adaptor-related protein complex 2, alpha 1 subunit|
|Calculated molecular weight||977 aa, 108 kDa|
|Observed molecular weight||108 kDa|
|GenBank accession number||BC014214|
|Gene ID (NCBI)||160|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.
|Product Specific Protocols|
|WB protocol for Alpha Adaptin antibody 11401-1-AP||Download protocol|
|IHC protocol for Alpha Adaptin antibody 11401-1-AP||Download protocol|
|IF protocol for Alpha Adaptin antibody 11401-1-AP||Download protocol|
|IP protocol for Alpha Adaptin antibody 11401-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Dig Dis Sci
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Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.
Ginsenoside compound-K inhibits the activity of B cells through inducing IgD-B cell receptor endocytosis in mice with collagen-induced arthritis.
Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis.
The AP-2 Clathrin Adaptor Mediates Endocytosis of an Inhibitory Killer Cell Ig-like Receptor in Human NK Cells.
Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.