Alpha Adaptin Polyclonal antibody

Alpha Adaptin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11401-1-AP

Synonyms

ADTAA, alpha Adaptin, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1



Tested Applications

Positive WB detected inHEK-293 cells, human heart tissue, human spleen tissue, mouse spleen tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11401-1-AP targets Alpha Adaptin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Adaptin fusion protein Ag1927
Full Name adaptor-related protein complex 2, alpha 1 subunit
Calculated molecular weight 977 aa, 108 kDa
Observed molecular weight 108 kDa
GenBank accession numberBC014214
Gene symbol AP2A1
Gene ID (NCBI) 160
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

Protocols

Product Specific Protocols
WB protocol for Alpha Adaptin antibody 11401-1-APDownload protocol
IHC protocol for Alpha Adaptin antibody 11401-1-APDownload protocol
IF protocol for Alpha Adaptin antibody 11401-1-APDownload protocol
IP protocol for Alpha Adaptin antibody 11401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dig Dis Sci

Speckle-Type POZ Protein Down-Regulates Matrix Metalloproteinase 2 Expression via Sp1/PI3K/Akt Signaling Pathway in Colorectal Cancer.

Authors - Shouhua Zhang
humanWB

Histopathology

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.

Authors - Ping Li
mouseIF

Inflammopharmacology

Ginsenoside compound-K inhibits the activity of B cells through inducing IgD-B cell receptor endocytosis in mice with collagen-induced arthritis.

Authors - Mei Zhang
humanWB

Neurobiol Dis

Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis.

Authors - George R Heaton
humanWB

J Immunol

The AP-2 Clathrin Adaptor Mediates Endocytosis of an Inhibitory Killer Cell Ig-like Receptor in Human NK Cells.

Authors - Amanda K Purdy

Cell Res

Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.

Authors - Ximing Shao