|Positive WB detected in||mouse liver tissue, HEK-293 cells|
|Positive IHC detected in||human liver tissue, human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
20578-1-AP targets APOB in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||apolipoprotein B (including Ag(x) antigen)|
|Calculated molecular weight||516 kDa|
|Observed molecular weight||130-150 kDa, 200-250 kDa, 400-520 kDa|
|GenBank accession number||NM_000384|
|Gene ID (NCBI)||338|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The apolipoprotein B (ApoB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The ApoB gene encodes two different isoproteins through mRNA editing, ApoB-48 and ApoB-100. ApoB-100 is synthesized exclusively by the liver and is essential for the assembly of VLDL in the liver. ApoB-48 is synthesized exclusively by the small intestine and is essential for the assembly of chylomicrons in the intestine. ApoB-48 and ApoB-100 share a common N-terminal sequence, but ApoB-48 lacks ApoB-100's C-terminal LDL receptor binding region. It is well established that ApoB-100 levels are associated with coronary heart disease. This antibody recognizes both of ApoB-48 and ApoB-100.
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