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ARAF Polyclonal antibody

ARAF Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22129-1-AP

Synonyms

A RAF, ARAF, ARAF1, PKS, PKS2, Proto oncogene A Raf, Proto oncogene A Raf 1, Proto oncogene Pks, RAFA1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ARAF Polyclonal antibody (22129-1-AP)

    WB analysis of HeLa using 22129-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ARAF Polyclonal antibody (22129-1-AP)

    WB analysis of A431 using 22129-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARAF Polyclonal antibody (22129-1-AP)

    WB analysis of NIH/3T3 using 22129-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using ARAF Polyclonal antibody (22129-1-AP)

    IHC staining of human skin cancer using 22129-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22129-1-AP (ARAF Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using ARAF Polyclonal antibody (22129-1-AP)

    IHC staining of human skin cancer using 22129-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22129-1-AP (ARAF Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, A431 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22129-1-AP is ARAF Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARAF fusion protein Ag17588
Full Name v-raf murine sarcoma 3611 viral oncogene homolog
Calculated Molecular Weight 609 aa, 68 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC007514
Gene Symbol ARAF
Gene ID (NCBI) 369
RRIDAB_2879001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).

Protocols

Product Specific Protocols
WB protocol for ARAF antibody 22129-1-APDownload protocol
IHC protocol for ARAF antibody 22129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncogene

Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.

Authors - Feifei Zhang
View Article
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article
mouseWB,IF

Oncol Lett

Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.

Authors - Ying Xu
View Article
humanWB

Front Med

5'-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I

Authors - Chengdong Wu
View Article