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  • KD/KO Validated

ARL15 Polyclonal antibody

ARL15 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11934-1-AP

Synonyms

ARF related protein 2, ARFRP2, ARL15



Tested Applications

Positive WB detected inmouse thymus tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11934-1-AP targets ARL15 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL15 fusion protein Ag2530
Full Name ADP-ribosylation factor-like 15
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC026093
Gene symbol ARL15
Gene ID (NCBI) 54622
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL15(ADP-ribosylation factor-like protein 15) is also named as ARFRP2(ADP-ribosylation factor-related protein 2) and belongs to the small GTPase superfamily and Arf familiy.ADP-ribosylation factor-related protein (ARP) is a membrane-associated GTPase with remote similarity to the family of ADP-ribosylation factors (ARF)(PMID:10092663). Arl15 is present in chordates but not lower eukaryotes. Arl15 lacks a site for Nmyristoylation, but the N-terminal region contains several well-conserved cysteines, suggesting that it may bepalmitoylated(PMID:17506703).

Protocols

Product Specific Protocols
WB protocol for ARL15 antibody 11934-1-APDownload protocol
IHC protocol for ARL15 antibody 11934-1-APDownload protocol
IP protocol for ARL15 antibody 11934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

PMID: 30682341

Authors - Jiayi Shen
  • KD Validated
humanIHC

Biochem Biophys Res Commun

ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT phosphorylation in the IR/IRS1/AKT pathway by interacting with ASAP2 and regulating PDPK1 activity.

Authors - Jie Zhao
humanWB

PLoS Genet

A genome-wide association study reveals variants in ARL15 that influence adiponectin levels.

Authors - Richards J Brent JB