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  • KD/KO Validated

ARPP-19 Polyclonal antibody

ARPP-19 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11678-1-AP

Synonyms

ARPP 16, ARPP 19, ARPP16, ARPP19, ARPP-19



Tested Applications

Positive WB detected inJurkat cells, A375 cells, A549 cells, mouse brain tissue, rat skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11678-1-AP targets ARPP-19 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPP-19 fusion protein Ag2279
Full Name cyclic AMP phosphoprotein, 19 kD
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC003418
Gene Symbol ARPP-19
Gene ID (NCBI) 10776
RRIDAB_2060078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARPP-19, a 19-kDa cAMP-regulated phosphoprotein, plays a role in regulating mitosis. Initiation and maintenance of mitosis require the activation of protein kinase cyclin B-Cdc2 and the inhibition of protein phosphatase 2A (PP2A). When phosphorylated by protein kinase Greatwall (Gwl), ARPP-19 associate with and inhibit PP2A, thus promoting mitotic entry. ARPP-19 may be an important link between nerve growth factor (NGF) signaling and post-transcriptional control of neuronal gene expression such as GAP-43.

Protocols

Product Specific Protocols
WB protocol for ARPP-19 antibody 11678-1-APDownload protocol
IHC protocol for ARPP-19 antibody 11678-1-APDownload protocol
IF protocol for ARPP-19 antibody 11678-1-APDownload protocol
IP protocol for ARPP-19 antibody 11678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Authors - Xigang Xia
humanWB

J Proteome Res

Phosphoproteome Profiling Revealed the Importance of mTOR Inhibition on CDK1 Activation to Further Regulate Cell Cycle Progression.

Authors - Luqi Jin
humanWB,IHC

Onco Targets Ther

PMID: 32753897

Authors - Xiang Gao
  • KD Validated
humanWB

Int J Mol Sci

Increased ARPP-19 expression is associated with hepatocellular carcinoma.

Authors - Haiyan Song
humanWB

Cancers (Basel)

Arpp19 Promotes Myc and Cip2a Expression and Associates with Patient Relapse in Acute Myeloid Leukemia.

Authors - Eleonora Mäkelä
  • KD Validated
humanWB,IP

Nature

Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19

Authors - Sathish K R Padi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (01-15-2021)

ARPP19 antibody giving a very clear sharp bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 for western
  • Cell Tissue Type: Human colon cancer cells