|Positive WB detected in||37°C incubated mouse heart tissue, 37°C incubated mouse skeletal muscle tissue|
|Positive IHC detected in||mouse heart tissue, human kidney tissue, human testis tissue, human skin tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16836-1-AP targets ATP1A2 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, Haliotis discus hannai, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP1A2 fusion protein Ag10515|
|Full Name||ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide|
|Calculated molecular weight||1020 aa, 112 kDa|
|Observed molecular weight||97-100 kDa|
|GenBank accession number||BC052271|
|Gene ID (NCBI)||477|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody was raised against the internal region of the human ATP1A2 and can recognizes all the isoforms of α subunit. The 65kDa band detected occasionally may be the degradation product of ATP1A2.
|Product Specific Protocols|
|WB protocol for ATP1A2 antibody 16836-1-AP||Download protocol|
|IHC protocol for ATP1A2 antibody 16836-1-AP||Download protocol|
|IF protocol for ATP1A2 antibody 16836-1-AP||Download protocol|
|FC protocol for ATP1A2 antibody 16836-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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