ATP1A2 Polyclonal antibody

ATP1A2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16836-1-AP

Synonyms

alpha 2 ATPase, ATP1A2, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2



Tested Applications

Positive WB detected in37°C incubated mouse heart tissue, 37°C incubated mouse skeletal muscle tissue
Positive IHC detected inhuman skin tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16836-1-AP targets ATP1A2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity abalones, canine, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A2 fusion protein Ag10515
Full Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Calculated molecular weight 1020 aa, 112 kDa
Observed molecular weight 97-100 kDa
GenBank accession numberBC052271
Gene symbol ATP1A2
Gene ID (NCBI) 477
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody was raised against the internal region of the human ATP1A2 and can recognizes all the isoforms of α subunit. The 65kDa band detected occasionally may be the degradation product of ATP1A2.

Protocols

Product Specific Protocols
WB protocol for ATP1A2 antibody 16836-1-APDownload protocol
IHC protocol for ATP1A2 antibody 16836-1-APDownload protocol
IF protocol for ATP1A2 antibody 16836-1-APDownload protocol
FC protocol for ATP1A2 antibody 16836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB

J Cereb Blood Flow Metab

Evaluating the involvement of cerebral microvascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter in electrolyte fluxes in an in vitro blood-brain barrier model of dehydration.

Authors - Kasper Lykke
humanWB

PLoS One

A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study.

Authors - Xin Liu
humanWB

Reproduction

Role of RAB5A in FSHR-mediated signal transduction in human granulosa cells.

Authors - Kai Zhu
humanIHC

Cancer Cell Int

Comprehensive analysis of the expression of sodium/potassium-ATPase α subunits and prognosis of ovarian serous cystadenocarcinoma.

Authors - Wei Huang
abalonesWB

Front Physiol

Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai.

Authors - Yanglei Jia
humanWB

Sci Rep

Elimination of undifferentiated human embryonic stem cells by cardiac glycosides.

Authors - Yu-Tsen Lin