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ATP5H Polyclonal antibody

ATP5H Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17589-1-AP

Synonyms

ATP5H, ATP5JD, ATPase subunit d, ATPQ

Validation Data Gallery

  • WB analysis of Jurkat using 17589-1-AP

    WB analysis of Jurkat using 17589-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17589-1-AP (ATP5H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 17589-1-AP

    IHC staining of human lung cancer using 17589-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17589-1-AP (ATP5H antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 17589-1-AP

    IF Staining of HepG2 using 17589-1-AP

    Immunofluorescent analysis of HepG2 cells, using ATP5H antibody 17589-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse liver using 17589-1-AP

    IP experiment of mouse liver using 17589-1-AP

    IP Result of anti-ATP5H (IP:17589-1-AP, 3ug; Detection:17589-1-AP 1:2000) with mouse liver tissue lysate 6000ug.

Tested Applications

Positive WB detected inJurkat cells, human brain tissue, human liver tissue, mouse brain tissue, mouse liver tissue, mouse ovary tissue, rat brain tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17589-1-AP targets ATP5H in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5H fusion protein Ag11429
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Calculated molecular weight 137 aa, 16 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC032245
Gene symbol ATP5H
Gene ID (NCBI) 10476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.

Protocols

Product Specific Protocols
WB protocol for ATP5H antibody 17589-1-APDownload protocol
IHC protocol for ATP5H antibody 17589-1-APDownload protocol
IF protocol for ATP5H antibody 17589-1-APDownload protocol
IP protocol for ATP5H antibody 17589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Zee (Verified Customer) (01-28-2020)

It worked well with western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue extract