ATP5H Antibody 17589-1-AP

Tested Applications

Applications:

ELISA, WB, IP, IHC, IF
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Jurkat cells were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human lung cancer using 17589-1-AP(ATP5H antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HepG2 cells, using ATP5H antibody 17589-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-ATP5H (IP:17589-1-AP, 3ug; Detection:17589-1-AP 1:2000) with mouse liver tissue lysate 6000ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

Jurkat cells, human brain tissue, human liver tissue, mouse brain tissue, mouse liver tissue, mouse ovary tissue, rat brain tissue, rat liver tissue

Positive IP detected in:

mouse liver tissue

Positive IHC detected in:

human lung cancer tissue, human lung cancer tissue, human pancreas tissue

Positive IF detected in:

HepG2 cells

Recommended dilution:

WB : 1:1000-1:6000

IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB

IHC : 1:20-1:200

IF : 1:10-1:100

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ATP5H fusion protein ag11429

Full name:

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d

Calculated molecular weight:

137aa,16 kDa

Observed molecular weight:

19-22 kDa

GenBank accession number:

BC032245

Gene ID (NCBI):

10476

Gene symbol

ATP5H

Synonyms

ATP5H, ATP5JD, ATPase subunit d, ATPQ

Background

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.

WB Figures

human brain tissue were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

human liver tissue were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

rat liver tissue were subjected to SDS PAGE followed by western blot with 17589-1-AP(ATP5H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human lung cancer using 17589-1-AP(ATP5H antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human lung cancer using 17589-1-AP(ATP5H antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 17589-1-AP( ATP5H Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 17589-1-AP( ATP5H Antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-ATP5H (IP:17589-1-AP, 3ug; Detection:17589-1-AP 1:2000) with mouse liver tissue lysate 6000ug.

IF Figures

Immunofluorescent analysis of HepG2 cells, using ATP5H antibody 17589-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ATP5H Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 17589-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [0] of [0] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

ATP5H Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 17589-1-AP

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Tested Applications

Product Information

Immunogen Information

Background