Tested Applications
| Positive WB detected in | Jurkat cells, mouse liver tissue, rat brain tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lung cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
17589-1-AP targets ATP5H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP5H fusion protein Ag11429 Predict reactive species |
| Full Name | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d |
| Calculated Molecular Weight | 137 aa, 16 kDa |
| Observed Molecular Weight | 19-22 kDa |
| GenBank Accession Number | BC032245 |
| Gene Symbol | ATP5H |
| Gene ID (NCBI) | 10476 |
| RRID | AB_2062046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75947 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP5H antibody 17589-1-AP | Download protocol |
| IHC protocol for ATP5H antibody 17589-1-AP | Download protocol |
| IF protocol for ATP5H antibody 17589-1-AP | Download protocol |
| IP protocol for ATP5H antibody 17589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy. | ||
Theranostics AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages. | ||
Brain Pathol Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus. | ||
Oncol Lett Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX | ||
J Nanobiotechnology Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia | ||
Science Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH SITING (Verified Customer) (05-25-2021) | good
 |
FH Zee (Verified Customer) (01-28-2020) | It worked well with western blot.
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