ATP5J Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 14114-1-AP

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Freight/Packing

Con: 20 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
human brain tissue, human heart tissue, mouse heart tissue, mouse liver tissue

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human osteosarcoma tissue

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6

Calculated molecular weight:
13 kDa

Observed molecular weight:
9 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ATP5J

Synonyms
ATP5, ATP5A, ATP5J, ATPase subunit F6, ATPM, CF6, F6
Background

ATP5J, also known as coupling factor 6 (CF6), is a soluble integral component of mitochondrial ATP synthase. Mitochondrial ATP synthase is a multi-subunit membrane-bound enzyme that catalyzes the synthesis of ATP by utilizing a proton electrochemical gradient. It consists of three domains, namely the extrinsic and intrinsic membrane domains (F1 and F0, respectively) joined by a stalk.CF6 is one of the subunits in the stalk and an essential component for energy transduction. Recently CF6 has also been reported to play a crucial role in the development of insulin resistance and hypertension. CF6 is first synthesized as an immature form in the cytosol, then transported to the mitochondria by an import signal peptide and becomes an active form with the signal peptide cleaved. Western blot analysis of CF6 demonstrates a single band around 9 kDa to 12 kDa in various tissues including heart, liver, brain and HUVEC (human umbilical vein endothelial cells).


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