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ATP6AP1 Polyclonal antibody

ATP6AP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15305-1-AP

Synonyms

16A, Ac45, ATP6AP1, ATP6IP1, ATP6S1, CF2, Protein XAP 3, V ATPase Ac45 subunit, V ATPase S1 accessory protein, V ATPase subunit S1, VATPS1, XAP 3, XAP3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using ATP6AP1 Polyclonal antibody (15305-1-AP)

    WB analysis of mouse kidney using 15305-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ATP6AP1 Polyclonal antibody (15305-1-AP)

    WB analysis of HepG2 using 15305-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATP6AP1 Polyclonal antibody (15305-1-AP)

    WB analysis of HeLa using 15305-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6AP1 Polyclonal antibody (15305-1-AP)

    IHC staining of human kidney using 15305-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6AP1 Polyclonal antibody (15305-1-AP)

    IHC staining of human kidney using 15305-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15305-1-AP targets ATP6AP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6AP1 fusion protein Ag7859
Full Name ATPase, H+ transporting, lysosomal accessory protein 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 46-52 kDa, 61-64 kDa
GenBank Accession NumberBC000724
Gene Symbol ATP6AP1
Gene ID (NCBI) 537
RRIDAB_10804756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATP6AP1 antibody 15305-1-APDownload protocol
IHC protocol for ATP6AP1 antibody 15305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Oncotarget

PMID: 28700346

Authors - Enrica Balza
View Article
humanWB

Proteomes

Integrative Proteomic and Phosphoproteomic Analyses of Hypoxia-Treated Pulmonary Artery Smooth Muscle Cells.

Authors - Ang Luo
View Article