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ATP6V1F Polyclonal antibody

ATP6V1F Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17725-1-AP

Synonyms

ATP6S14, ATP6V1F, V ATPase 14 kDa subunit, V ATPase subunit F, V type proton ATPase subunit F, Vacuolar proton pump subunit F, VATF, Vma7

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ATP6V1F Polyclonal antibody (17725-1-AP)

    WB analysis of MCF-7 using 17725-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATP6V1F Polyclonal antibody (17725-1-AP)

    WB analysis of HeLa using 17725-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ATP6V1F Polyclonal antibody (17725-1-AP)

    WB analysis of Jurkat using 17725-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    WB analysis of mouse skeletal muscle using 17725-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    WB analysis of mouse liver using 17725-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17725-1-AP (ATP6V1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human testis using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human testis using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human brain using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human brain using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human pancreas using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17725-1-AP (ATP6V1F Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human pancreas using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17725-1-AP (ATP6V1F Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human kidney using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human kidney using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human placenta using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ATP6V1F Polyclonal antibody (17725-1-AP)

    IHC staining of human placenta using 17725-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17725-1-AP (ATP6V1F antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of MCF-7 cells using ATP6V1F Polyclonal antibody (17725-1-AP)

    FC experiment of MCF-7 using 17725-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug ATP6V1F antibody (17725-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, Jurkat cells, mouse liver tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human pancreas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17725-1-AP targets ATP6V1F in WB, IHC, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1F fusion protein Ag12121
Full Name ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
Calculated Molecular Weight 119 aa, 13 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC107854
Gene Symbol ATP6V1F
Gene ID (NCBI) 9296
RRIDAB_2062680
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa.

Protocols

Product Specific Protocols
WB protocol for ATP6V1F antibody 17725-1-APDownload protocol
IHC protocol for ATP6V1F antibody 17725-1-APDownload protocol
FC protocol for ATP6V1F antibody 17725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

BMC Med Genomics

ATP6V1F is a novel prognostic biomarker and potential immunotherapy target for hepatocellular carcinoma

Authors - Xinyao Hu
View Article
humanWB,CoIP

J Proteome Res

Proteomic Investigation of Differential Interactomes of Glypican 1 and a Putative Disease-Modifying Variant of Ataxia

Authors - Nur Cengiz Winter
View Article
humanWB

Front Oncol

Genome-wide association studies identify miRNA-194 as a prognostic biomarker for gastrointestinal cancer by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5

Authors - Pan Huang
View Article