CD80/B7-1 Polyclonal antibody

CD80/B7-1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse


WB, IHC, IF, FC, Cell treatment, ELISA



Cat no : 14292-1-AP


Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CTLA 4 counter receptor B7.1, LAB7

Tested Applications

Positive WB detected inHuman peripheral blood leukocyte, RAW 264.7 cells, mouse thymus tissue, mouse skeletal muscle tissue
Positive FC detected inRaji cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

14292-1-AP targets CD80/B7-1 in WB, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD80/B7-1 fusion protein Ag5615
Full Name CD80 molecule
Calculated molecular weight 33 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC042665
Gene symbol CD80
Gene ID (NCBI) 941
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)


Product Specific Protocols
WB protocol for CD80/B7-1 antibody 14292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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