Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

1X10^6 Raji cells were stained with 0.2ug CD80/B7-1 antibody (14292-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.