CD80/B7-1 Polyclonal antibody

CD80/B7-1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 14292-1-AP

Synonyms

Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CTLA 4 counter receptor B7.1, LAB7



Tested Applications

Positive WB detected inRaji cells, RAW 264.7 cells, human peripheral blood leukocyte, Ramos cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14292-1-AP targets CD80/B7-1 in WB, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD80/B7-1 fusion protein Ag5615
Full Name CD80 molecule
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC042665
Gene Symbol CD80
Gene ID (NCBI) 941
RRIDAB_10640809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocols

Product Specific Protocols
WB protocol for CD80/B7-1 antibody 14292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

ACS Appl Mater Interfaces

Fe(III)-Chelated Polydopamine Nanoparticles for Synergistic Tumor Therapies of Enhanced Photothermal Ablation and Antitumor Immune Activation.

Authors - Na Xu
mouseFC

Biomaterials

Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.

Authors - Haoran Wang
mouseFC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
mouse

Int J Nanomedicine

Self-Assembly Catalase Nanocomplex Conveyed by Bacterial Vesicles for Oxygenated Photodynamic Therapy and Tumor Immunotherapy.

Authors - Jiayu Zhang
mouseIHC

J Nanobiotechnology

Continuous ZnO nanoparticle exposure induces melanoma-like skin lesions in epidermal barrier dysfunction model mice through anti-apoptotic effects mediated by the oxidative stress-activated NF-κB pathway.

Authors - Ping Wang
mouseFC

Drug Deliv

Sonosensitizer nanoplatform-mediated sonodynamic therapy induced immunogenic cell death and tumor immune microenvironment variation.

Authors - Jing Zheng