CD80/B7-1 Polyclonal antibody

Name: CD80/B7-1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 14292-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 14292-1-AP

Synonyms

Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CTLA 4 counter receptor B7.1, LAB7

WB analysis of human peripheral blood leukocyte using 14292-1-AP
Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
FC experiment of Raji using 14292-1-AP
1X10^6 Raji cells were stained with 0.2ug CD80/B7-1 antibody (14292-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Cat No. 14292-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Positive WB detected in	Human peripheral blood leukocyte, RAW 264.7 cells, mouse thymus tissue, mouse skeletal muscle tissue
Positive FC detected in	Raji cells

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

WB	See 1 publications below
FC	See 1 publications below

14292-1-AP targets CD80/B7-1 in WB, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD80/B7-1 fusion protein Ag5615
Full Name	CD80 molecule
Calculated molecular weight	33 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC042665
Gene symbol	CD80
Gene ID (NCBI)	941
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for CD80/B7-1 antibody 14292-1-AP	Download protocol
FC protocol for CD80/B7-1 antibody 14292-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB	Virulence Differential immune responses of C57BL/6 mice to infection by Salmonella enterica serovar Typhimurium strain SL1344, CVCC541 and CMCC50115. Authors - Shao Wei View Article
mouse	FC	Biomaterials Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia. Authors - Haoran Wang View Article