|Positive WB detected in||mouse brain tissue, human brain tissue, mouse pancreas tissue, HeLa cells, zebrafish tissue, PC-12 cells, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
12807-1-AP targets BACE1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.
|Tested Reactivity||human, mouse, zebrafish, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BACE1 fusion protein Ag3559|
|Full Name||beta-site APP-cleaving enzyme 1|
|Calculated molecular weight||501 aa, 56 kDa|
|Observed molecular weight||70-75 kDa, 42-56 kDa|
|GenBank accession number||BC036084|
|Gene ID (NCBI)||23621|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).
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