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BAG1S/1M/1L Polyclonal antibody

BAG1S/1M/1L Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16150-1-AP

Synonyms



Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells, mouse liver tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16150-1-AP targets BAG1S/1M/1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name BCL2-associated athanogene
Calculated molecular weight 26 kDa
Observed molecular weight 46-50 kDa, 41-46 kDa, 25-33 kDa
GenBank accession numberNM_004323
Gene symbol BAG1
Gene ID (NCBI) 573
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recongizes all the isoforms of BAG1, includes GAG1L, BAG1V, BAG1M and BAG1S.

Protocols

Product Specific Protocols
WB protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
IHC protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Tumour Biol

Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.

Authors - Wenbai Huang
  • KD Validated