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  • KD/KO Validated

BAG3 Polyclonal antibody

BAG3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CO-IP, ELISA

Conjugate

Unconjugated

Cat no : 10599-1-AP

Synonyms

BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, mouse heart, rat heart
Positive IP detected inK-562 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10599-1-AP targets BAG3 in WB, IP, IHC, IF, CO-IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG3 fusion protein Ag0956
Full Name BCL2-associated athanogene 3
Calculated molecular weight 61 kDa
Observed molecular weight 74-80 kDa
GenBank accession numberBC006418
Gene symbol BAG3
Gene ID (NCBI) 9531
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.

Protocols

Product Specific Protocols
WB protocol for BAG3 antibody 10599-1-APDownload protocol
IHC protocol for BAG3 antibody 10599-1-APDownload protocol
IF protocol for BAG3 antibody 10599-1-APDownload protocol
IP protocol for BAG3 antibody 10599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Autophagy

BAG3 and SYNPO (synaptopodin) facilitate phospho-MAPT/Tau degradation via autophagy in neuronal processes.

Authors - Changyi Ji
  • KD Validated
mouseWB,IF

Redox Biol

Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress.

Authors - Debapriya Chakraborty
  • KD Validated
ratWB,IF

Sci Rep

Role of Bcl2-associated Athanogene 3 in Turnover of Gap Junction Protein, Connexin 43, in Neonatal Cardiomyocytes.

Authors - Farzaneh Ghasemi Tahrir
  • KD Validated
WB,IF

Cell Death Dis

Perturbation of synapsins homeostasis through HIV-1 Tat-mediated suppression of BAG3 in primary neuronal cells.

Authors - Taha Mohseni Ahooyi
  • KD Validated
mouseWB,IF

Neurobiol Aging

Nrf2 mediates the expression of BAG3 and autophagy cargo adaptor proteins and tau clearance in an age-dependent manner.

Authors - Maoping Tang
humanWB

J Biol Chem

Silencing of the Hsp70-specific nucleotide-exchange factor BAG3 corrects the F508del-CFTR variant by restoring autophagy.

Authors - Darren M Hutt
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This bag3 antibody is very specific without any non-specific bands. It is the best Bag3 antibody out there for western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
BAG3 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:10599-1-AP)
FH

Afaque (Verified Customer) (10-17-2019)

I have used this antibody to study autophagy. It has worked very well for my Western blots. I also tried for IF and got very good results.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Cardiomyocytes
BAG3 Antibody Western Blot,Immunofluorescence, validation (1:3000 dilution) in Cardiomyocytes (Cat no:10599-1-AP)
FH

SHUBHAM (Verified Customer) (02-25-2019)

excellent Antibody

  • Applications: Western Blot,Immunohistochemistry,Cell culture,
  • Cell Tissue Type: AC16
BAG3 Antibody Western Blot,Immunohistochemistry,Cell culture, validation ( dilution) in AC16 (Cat no:10599-1-AP)
FH

Praveen (Verified Customer) (12-03-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Brain,(Rat), Heart, Skeletal muscles, lung, Kidney etc (Mice), Human Cardio myocytes