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  • KD/KO Validated

DDX39B Polyclonal antibody

DDX39B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14798-1-AP

Synonyms

ATP dependent RNA helicase p47, BAT1, D6S81E, DDX39, DDX39B, DEAD box protein UAP56, HLA B associated transcript 1, Spliceosome RNA helicase BAT1, UAP56



Tested Applications

Positive WB detected inJurkat cells, rat spleen tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14798-1-AP targets DDX39B in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX39B fusion protein Ag6512
Full Name HLA-B associated transcript 1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000361
Gene Symbol BAT1
Gene ID (NCBI) 7919
RRIDAB_2061854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.

Protocols

Product Specific Protocols
WB protocol for DDX39B antibody 14798-1-APDownload protocol
IHC protocol for DDX39B antibody 14798-1-APDownload protocol
IP protocol for DDX39B antibody 14798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
humanIF,ChIP

Genes Dev

UAP56/DDX39B is a major cotranscriptional RNA-DNA helicase that unwinds harmful R loops genome-wide.

Authors - Carmen Pérez-Calero
human,mouseWB,IHC

Cell Death Discov

DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1.

Authors - Haonan Zhang
  • KD Validated
humanWB

iScience

THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate

Authors - Mareike Polenkowski
pigWB

ACS Infect Dis

Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.

Authors - Lin Yang
humanWB,IF,IP

Sci Rep

ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.

Authors - Ashley Boehringer