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  • KD/KO Validated

BCCIP Polyclonal antibody

BCCIP Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16043-1-AP

Synonyms

BCCIP, Protein TOK 1, TOK 1, TOK1



Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16043-1-AP targets BCCIP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCCIP fusion protein Ag8934
Full Name BRCA2 and CDKN1A interacting protein
Calculated molecular weight 314 aa, 36 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC009771
Gene symbol BCCIP
Gene ID (NCBI) 56647
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.

Protocols

Product Specific Protocols
WB protocol for BCCIP antibody 16043-1-APDownload protocol
IF protocol for BCCIP antibody 16043-1-APDownload protocol
IP protocol for BCCIP antibody 16043-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Int J Mol Sci

YY1/BCCIP Coordinately Regulates P53-Responsive Element (p53RE)-Mediated Transactivation of p21Waf1/Cip1.

Authors - Yi Sui
  • KD Validated
humanWB,IHC

Int J Oncol

Differential BCCIP gene expression in primary human ovarian cancer, renal cell carcinoma and colorectal cancer tissues.

Authors - Liu Xiaoxia X
humanWB

Biochem Biophys Res Commun

BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma.

Authors - Fang Wang
humanWB,IF

Protein Cell

Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells.

Authors - Jiaming Su
humanWB,IF

FEBS J

BCCIP binds to and activates its promoter in a YY1-dependent fashion in HCT116 cells.

Authors - Yi Sui
  • KD Validated